Gene description for ATP5S
Gene name ATP synthase, H+ transporting, mitochondrial Fo complex, subunit s (factor B)
Gene symbol ATP5S
Other names/aliases ATPW
FB
HSU79253
Species Homo sapiens
 Database cross references - ATP5S
ExoCarta ExoCarta_27109
Vesiclepedia VP_27109
Entrez Gene 27109
HGNC 18799
UniProt Q99766  
 ATP5S identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ATP5S
Molecular Function
    protein binding GO:0005515 IPI
    proton transmembrane transporter activity GO:0015078 NAS
    metal ion binding GO:0046872 IEA
Biological Process
    ATP biosynthetic process GO:0006754 IEA
    proton transmembrane transport GO:1902600 NAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HTP
    mitochondrial inner membrane GO:0005743 TAS
    proton-transporting ATP synthase complex, coupling factor F(o) GO:0045263 IEA
 Experiment description of studies that identified ATP5S in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ATP5S
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ING3  
Affinity Capture-MS Homo sapiens
2 KLC3  
Affinity Capture-MS Homo sapiens
3 SPINK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 KLHL38  
Two-hybrid Homo sapiens
5 LLGL2 3993
Affinity Capture-MS Homo sapiens
6 KIF2A 3796
Affinity Capture-MS Homo sapiens
7 Tnks  
Affinity Capture-MS Mus musculus
8 RNASEH2B  
Affinity Capture-MS Homo sapiens
9 TRMT2A 27037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 THOC2 57187
Affinity Capture-MS Homo sapiens
11 MYH13 8735
Affinity Capture-MS Homo sapiens
12 PPIA 5478
Affinity Capture-MS Homo sapiens
13 MYO5C 55930
Affinity Capture-MS Homo sapiens
14 CCL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 EVX2  
Two-hybrid Homo sapiens
16 Sesn2  
Affinity Capture-MS Mus musculus
17 MFSD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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