Gene description for Ahcy
Gene name S-adenosylhomocysteine hydrolase
Gene symbol Ahcy
Other names/aliases AA987153
AL024110
CuBP
SAHH
Species Mus musculus
 Database cross references - Ahcy
ExoCarta ExoCarta_269378
Vesiclepedia VP_269378
Entrez Gene 269378
UniProt P50247  
 Ahcy identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ahcy
Molecular Function
    adenosylhomocysteinase activity GO:0004013 IBA
    adenosylhomocysteinase activity GO:0004013 IDA
    adenosylhomocysteinase activity GO:0004013 ISO
    copper ion binding GO:0005507 IDA
    protein binding GO:0005515 IPI
    adenyl nucleotide binding GO:0030554 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    NAD binding GO:0051287 IDA
    NAD binding GO:0051287 ISO
Biological Process
    chronic inflammatory response to antigenic stimulus GO:0002439 ISO
    one-carbon metabolic process GO:0006730 IEA
    response to nutrient GO:0007584 ISO
    S-adenosylhomocysteine catabolic process GO:0019510 IDA
    S-adenosylhomocysteine catabolic process GO:0019510 ISO
    S-adenosylmethionine cycle GO:0033353 IBA
    circadian sleep/wake cycle GO:0042745 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    melanosome GO:0042470 IEA
 Experiment description of studies that identified Ahcy in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ahcy
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
2 Fzr1  
Affinity Capture-MS Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 Tfe3  
Affinity Capture-MS Mus musculus
5 Atg16l1  
Affinity Capture-MS Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
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