Gene description for Gphn
Gene name gephyrin
Gene symbol Gphn
Other names/aliases 5730552E08Rik
AI662856
BC027112
C230040D23
GPH
GPHRYN
geph
Species Mus musculus
 Database cross references - Gphn
ExoCarta ExoCarta_268566
Vesiclepedia VP_268566
Entrez Gene 268566
UniProt Q8BUV3  
 Gphn identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gphn
Molecular Function
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    cytoskeletal protein binding GO:0008092 TAS
    nitrate reductase activity GO:0008940 ISO
    identical protein binding GO:0042802 ISO
    identical protein binding GO:0042802 ISS
    molybdopterin cofactor binding GO:0043546 ISO
    metal ion binding GO:0046872 IEA
    molecular adaptor activity GO:0060090 ISO
    molybdopterin adenylyltransferase activity GO:0061598 ISO
    molybdopterin molybdotransferase activity GO:0061599 IBA
    molybdopterin molybdotransferase activity GO:0061599 IMP
    molybdopterin molybdotransferase activity GO:0061599 ISO
Biological Process
    Mo-molybdopterin cofactor biosynthetic process GO:0006777 IBA
    Mo-molybdopterin cofactor biosynthetic process GO:0006777 IMP
    Mo-molybdopterin cofactor biosynthetic process GO:0006777 ISO
    establishment of synaptic specificity at neuromuscular junction GO:0007529 IBA
    establishment of synaptic specificity at neuromuscular junction GO:0007529 IMP
    response to metal ion GO:0010038 ISO
    molybdopterin cofactor biosynthetic process GO:0032324 IMP
    molybdopterin cofactor biosynthetic process GO:0032324 ISO
    molybdopterin cofactor biosynthetic process GO:0032324 ISS
    establishment of protein localization GO:0045184 ISO
    glycine receptor clustering GO:0072579 IBA
    glycine receptor clustering GO:0072579 IMP
    gamma-aminobutyric acid receptor clustering GO:0097112 IBA
    gamma-aminobutyric acid receptor clustering GO:0097112 IGI
    gamma-aminobutyric acid receptor clustering GO:0097112 ISO
    gamma-aminobutyric acid receptor clustering GO:0097112 ISS
    postsynaptic neurotransmitter receptor diffusion trapping GO:0098970 IBA
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 IDA
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 IMP
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 ISO
Subcellular Localization
    intracellular anatomical structure GO:0005622 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytoskeleton GO:0005856 IEA
    cytoplasmic side of plasma membrane GO:0009898 ISO
    postsynaptic density GO:0014069 IDA
    extrinsic component of plasma membrane GO:0019897 TAS
    dendrite GO:0030425 IBA
    dendrite GO:0030425 IDA
    dendrite GO:0030425 ISO
    dendrite GO:0030425 ISS
    neuronal cell body GO:0043025 IDA
    postsynaptic membrane GO:0045211 IBA
    postsynaptic membrane GO:0045211 ISO
    postsynaptic membrane GO:0045211 ISS
    inhibitory synapse GO:0060077 IDA
    inhibitory synapse GO:0060077 ISO
    synaptic membrane GO:0097060 IDA
    synaptic membrane GO:0097060 ISO
    glycinergic synapse GO:0098690 ISO
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 ISO
    GABA-ergic synapse GO:0098982 IDA
    GABA-ergic synapse GO:0098982 IDA
    GABA-ergic synapse GO:0098982 IMP
    GABA-ergic synapse GO:0098982 ISO
    postsynaptic specialization, intracellular component GO:0099091 IDA
    postsynaptic specialization, intracellular component GO:0099091 ISO
    postsynaptic specialization GO:0099572 IBA
    postsynaptic specialization GO:0099572 ISO
 Experiment description of studies that identified Gphn in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gphn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Reconstituted Complex Mus musculus
2 Gabra1  
Affinity Capture-MS Mus musculus
3 Hcn1  
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Agap2  
Affinity Capture-MS Mus musculus
7 Huwe1  
Co-localization Mus musculus
8 Glrb  
Affinity Capture-Western Rattus norvegicus
9 Gfm2  
Co-fractionation Mus musculus
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