Gene description for Adgre5
Gene name adhesion G protein-coupled receptor E5
Gene symbol Adgre5
Other names/aliases AA409984
Cd97
TM7LN1
Species Mus musculus
 Database cross references - Adgre5
ExoCarta ExoCarta_26364
Vesiclepedia VP_26364
Entrez Gene 26364
UniProt Q9Z0M6  
 Adgre5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Adgre5
Molecular Function
    transmembrane signaling receptor activity GO:0004888 TAS
    G protein-coupled receptor activity GO:0004930 IBA
    G protein-coupled receptor activity GO:0004930 IEA
    calcium ion binding GO:0005509 IEA
    chondroitin sulfate binding GO:0035374 ISO
Biological Process
    cell adhesion GO:0007155 IEA
    cell surface receptor signaling pathway GO:0007166 IEA
    G protein-coupled receptor signaling pathway GO:0007186 IEA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    positive regulation of synapse assembly GO:0051965 IDA
Subcellular Localization
    extracellular region GO:0005576 IEA
    plasma membrane GO:0005886 IBA
    external side of plasma membrane GO:0009897 IDA
    membrane GO:0016020 TAS
    leading edge membrane GO:0031256 ISO
 Experiment description of studies that identified Adgre5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Adgre5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Scube1  
Co-fractionation Mus musculus
2 Atp6ap1  
Co-fractionation Mus musculus
3 Lama5 16776
Co-fractionation Mus musculus
4 Col5a1 12831
Co-fractionation Mus musculus
5 Man2b1 17159
Co-fractionation Mus musculus
6 Nell2  
Co-fractionation Mus musculus
7 Ltbp1 268977
Co-fractionation Mus musculus
8 Ltbp3  
Co-fractionation Mus musculus
9 Dnase2a  
Co-fractionation Mus musculus
10 Sumf1  
Co-fractionation Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
12 Fbn1  
Co-fractionation Mus musculus
13 Plxnb2 140570
Co-fractionation Mus musculus
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