Gene description for TTLL3
Gene name tubulin tyrosine ligase-like family member 3
Gene symbol TTLL3
Other names/aliases HOTTL
Species Homo sapiens
 Database cross references - TTLL3
ExoCarta ExoCarta_26140
Vesiclepedia VP_26140
Entrez Gene 26140
HGNC 24483
UniProt Q9Y4R7  
 TTLL3 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for TTLL3
Molecular Function
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
    protein-glycine ligase activity GO:0070735 IDA
    protein-glycine ligase activity GO:0070735 TAS
    protein-glycine ligase activity, initiating GO:0070736 IBA
    protein-glycine ligase activity, initiating GO:0070736 ISS
    tubulin-glycine ligase activity GO:0070738 IEA
Biological Process
    spermatogenesis GO:0007283 IBA
    protein polyglycylation GO:0018094 IDA
    flagellated sperm motility GO:0030317 IBA
    flagellated sperm motility GO:0030317 ISS
    axoneme assembly GO:0035082 IBA
    axoneme assembly GO:0035082 ISS
    cilium assembly GO:0060271 ISS
Subcellular Localization
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    cilium GO:0005929 ISS
    axoneme GO:0005930 IBA
    axoneme GO:0005930 ISS
    microtubule cytoskeleton GO:0015630 IBA
    microtubule cytoskeleton GO:0015630 ISS
    sperm flagellum GO:0036126 IBA
    sperm flagellum GO:0036126 ISS
 Experiment description of studies that identified TTLL3 in exosomes
1
Experiment ID 79
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for TTLL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDCL 5082
Affinity Capture-MS Homo sapiens
2 CDCA5  
Two-hybrid Homo sapiens
3 DAPK1 1612
Affinity Capture-MS Homo sapiens
4 ZNHIT2 741
Affinity Capture-MS Homo sapiens
5 SUFU 51684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CCT7 10574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TCP1 6950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HSPA1L 3305
Affinity Capture-MS Homo sapiens
9 CCT6A 908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CCT3 7203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CCT4 10575
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CCT6B 10693
Affinity Capture-MS Homo sapiens
13 ARPC4-TTLL3 100526693
Affinity Capture-MS Homo sapiens
14 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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