Gene description for Krt8
Gene name keratin 8, type II
Gene symbol Krt8
Other names/aliases CYKER
Krt2-8
Species Rattus norvegicus
 Database cross references - Krt8
ExoCarta ExoCarta_25626
Vesiclepedia VP_25626
Entrez Gene 25626
UniProt Q10758  
 Krt8 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Krt8
Molecular Function
    protein binding GO:0005515 IPI
    protein-containing complex binding GO:0044877 IPI
    scaffold protein binding GO:0097110 ISO
Biological Process
    tumor necrosis factor-mediated signaling pathway GO:0033209 ISO
    sarcomere organization GO:0045214 IDA
    response to hydrostatic pressure GO:0051599 IDA
    response to other organism GO:0051707 ISO
    cell differentiation involved in embryonic placenta development GO:0060706 ISO
    extrinsic apoptotic signaling pathway GO:0097191 ISO
    hepatocyte apoptotic process GO:0097284 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    intermediate filament GO:0005882 ISO
    cell-cell junction GO:0005911 ISO
    dystrophin-associated glycoprotein complex GO:0016010 IDA
    apicolateral plasma membrane GO:0016327 ISO
    nuclear matrix GO:0016363 IEA
    Z disc GO:0030018 IDA
    Z disc GO:0030018 ISO
    sarcolemma GO:0042383 IDA
    sarcolemma GO:0042383 ISO
    costamere GO:0043034 IDA
    keratin filament GO:0045095 IDA
    keratin filament GO:0045095 IEA
    keratin filament GO:0045095 ISO
    intermediate filament cytoskeleton GO:0045111 ISO
    cell periphery GO:0071944 ISO
 Experiment description of studies that identified Krt8 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Krt8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STXBP5L  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Krt8 is involved
PathwayEvidenceSource
Developmental Biology IEA Reactome
Formation of the cornified envelope IEA Reactome
Keratinization IEA Reactome





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