Gene description for Cd81
Gene name Cd81 molecule
Gene symbol Cd81
Other names/aliases Tapa1
Species Rattus norvegicus
 Database cross references - Cd81
ExoCarta ExoCarta_25621
Vesiclepedia VP_25621
Entrez Gene 25621
UniProt Q62745  
 Cd81 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 19367702    
Serum 33159193    
 Gene ontology annotations for Cd81
Molecular Function
    virus receptor activity GO:0001618 IEA
    virus receptor activity GO:0001618 ISO
    integrin binding GO:0005178 IEA
    integrin binding GO:0005178 ISO
    integrin binding GO:0005178 ISS
    protein binding GO:0005515 IPI
    cholesterol binding GO:0015485 IEA
    cholesterol binding GO:0015485 ISO
    cholesterol binding GO:0015485 ISS
    MHC class II protein binding GO:0042289 IEA
    MHC class II protein binding GO:0042289 ISO
    transferrin receptor binding GO:1990459 IEA
    transferrin receptor binding GO:1990459 ISO
Biological Process
    immunological synapse formation GO:0001771 IEA
    immunological synapse formation GO:0001771 ISO
    immunological synapse formation GO:0001771 ISS
    humoral immune response mediated by circulating immunoglobulin GO:0002455 IEA
    humoral immune response mediated by circulating immunoglobulin GO:0002455 ISO
    humoral immune response mediated by circulating immunoglobulin GO:0002455 ISS
    positive regulation of inflammatory response to antigenic stimulus GO:0002863 ISO
    positive regulation of inflammatory response to antigenic stimulus GO:0002863 ISS
    myoblast fusion involved in skeletal muscle regeneration GO:0014905 ISO
    myoblast fusion involved in skeletal muscle regeneration GO:0014905 ISS
    positive regulation of B cell proliferation GO:0030890 IEA
    positive regulation of B cell proliferation GO:0030890 ISO
    receptor internalization GO:0031623 ISO
    receptor internalization GO:0031623 ISS
    regulation of protein stability GO:0031647 IEA
    regulation of protein stability GO:0031647 ISO
    macrophage fusion GO:0034238 IEA
    macrophage fusion GO:0034238 ISO
    macrophage fusion GO:0034238 ISS
    CD4-positive, alpha-beta T cell costimulation GO:0035783 IEA
    CD4-positive, alpha-beta T cell costimulation GO:0035783 ISO
    CD4-positive, alpha-beta T cell costimulation GO:0035783 ISS
    regulation of growth GO:0040008 IEP
    regulation of cell population proliferation GO:0042127 IDA
    positive regulation of MAPK cascade GO:0043410 IEA
    positive regulation of MAPK cascade GO:0043410 ISO
    positive regulation of MAPK cascade GO:0043410 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    symbiont entry into host cell GO:0046718 IEA
    positive regulation of B cell receptor signaling pathway GO:0050861 IEA
    positive regulation of B cell receptor signaling pathway GO:0050861 ISO
    positive regulation of B cell receptor signaling pathway GO:0050861 ISS
    positive regulation of T cell receptor signaling pathway GO:0050862 IEA
    positive regulation of T cell receptor signaling pathway GO:0050862 ISO
    positive regulation of T cell receptor signaling pathway GO:0050862 ISS
    positive regulation of B cell activation GO:0050871 ISO
    positive regulation of B cell activation GO:0050871 ISS
    protein localization to lysosome GO:0061462 IEA
    protein localization to lysosome GO:0061462 ISO
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 IEA
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 ISO
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 ISS
    cellular response to low-density lipoprotein particle stimulus GO:0071404 ISO
    cellular response to low-density lipoprotein particle stimulus GO:0071404 ISS
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    protein localization to plasma membrane GO:0072659 ISS
    osteoclast fusion GO:0072675 ISO
    osteoclast fusion GO:0072675 ISS
    positive regulation of receptor clustering GO:1903911 IEA
    positive regulation of receptor clustering GO:1903911 ISO
    positive regulation of receptor clustering GO:1903911 ISS
    positive regulation of protein catabolic process in the vacuole GO:1904352 IEA
    positive regulation of protein catabolic process in the vacuole GO:1904352 ISO
    regulation of macrophage migration GO:1905521 ISO
    regulation of macrophage migration GO:1905521 ISS
    positive regulation of adaptive immune memory response GO:1905676 IEA
    positive regulation of adaptive immune memory response GO:1905676 ISO
    regulation of cell motility GO:2000145 ISO
    positive regulation of T-helper 2 cell cytokine production GO:2000553 IEA
    positive regulation of T-helper 2 cell cytokine production GO:2000553 ISO
    positive regulation of T-helper 2 cell cytokine production GO:2000553 ISS
    positive regulation of CD4-positive, alpha-beta T cell proliferation GO:2000563 IEA
    positive regulation of CD4-positive, alpha-beta T cell proliferation GO:2000563 ISO
    positive regulation of CD4-positive, alpha-beta T cell proliferation GO:2000563 ISS
    positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell GO:2001190 IEA
    positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell GO:2001190 ISO
    positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell GO:2001190 ISS
Subcellular Localization
    immunological synapse GO:0001772 IEA
    immunological synapse GO:0001772 ISO
    immunological synapse GO:0001772 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    basal plasma membrane GO:0009925 ISO
    membrane GO:0016020 ISO
    basolateral plasma membrane GO:0016323 IEA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 IDA
    vesicle GO:0031982 ISO
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    tetraspanin-enriched microdomain GO:0097197 IEA
    tetraspanin-enriched microdomain GO:0097197 ISO
    tetraspanin-enriched microdomain GO:0097197 ISS
 Experiment description of studies that identified Cd81 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
6
Experiment ID 323
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33159193    
Organism Rattus norvegicus
Experiment description Treatment of infarcted heart tissue via the capture and local delivery of circulating exosomes through antibody-conjugated magnetic nanoparticles
Authors "Liu S, Chen X, Bao L, Liu T, Yuan P, Yang X, Qiu X, Gooding JJ, Bai Y, Xiao J, Pu F, Jin Y. "
Journal name Nat Biomed Eng
Publication year 2020
Sample Serum
Sample name Serum - Sprague Dawley rats
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for Cd81
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STXBP5L  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Cd81 is involved
No pathways found





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