Gene description for Rpn1
Gene name ribophorin I
Gene symbol Rpn1
Other names/aliases RIBI
RPN-I
Species Rattus norvegicus
 Database cross references - Rpn1
ExoCarta ExoCarta_25596
Vesiclepedia VP_25596
Entrez Gene 25596
 Rpn1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Rpn1
Biological Process
    protein glycosylation GO:0006486 IEA
    protein glycosylation GO:0006486 ISO
    protein N-linked glycosylation GO:0006487 ISO
    peptide transport GO:0015833 NAS
    protein N-linked glycosylation via asparagine GO:0018279 IBA
    protein N-linked glycosylation via asparagine GO:0018279 IEA
    protein N-linked glycosylation via asparagine GO:0018279 ISO
Subcellular Localization
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    rough endoplasmic reticulum GO:0005791 IEA
    rough endoplasmic reticulum GO:0005791 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    oligosaccharyltransferase complex GO:0008250 IBA
    oligosaccharyltransferase complex GO:0008250 IEA
    oligosaccharyltransferase complex GO:0008250 ISO
 Experiment description of studies that identified Rpn1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Rpn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rad23b  
Affinity Capture-MS Rattus norvegicus
2 Psmd4  
Affinity Capture-Western Rattus norvegicus
3 Fbxo21  
Affinity Capture-MS Rattus norvegicus
4 Sumo3  
Affinity Capture-MS Rattus norvegicus
5 Ublcp1  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Rpn1 is involved
No pathways found





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