Gene description for Acsl1
Gene name acyl-CoA synthetase long-chain family member 1
Gene symbol Acsl1
Other names/aliases ACS
Acas
COAA
Facl2
Species Rattus norvegicus
 Database cross references - Acsl1
ExoCarta ExoCarta_25288
Vesiclepedia VP_25288
Entrez Gene 25288
UniProt P18163  
 Acsl1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Acsl1
Molecular Function
    acetate-CoA ligase activity GO:0003987 TAS
    long-chain fatty acid-CoA ligase activity GO:0004467 IDA
    long-chain fatty acid-CoA ligase activity GO:0004467 IEA
    long-chain fatty acid-CoA ligase activity GO:0004467 ISO
    ATP binding GO:0005524 IEA
    protein serine/threonine kinase activator activity GO:0043539 ISO
    arachidonate-CoA ligase activity GO:0047676 IBA
    arachidonate-CoA ligase activity GO:0047676 IDA
    phytanate-CoA ligase activity GO:0050197 IDA
    pristanate-CoA ligase activity GO:0070251 IEA
    oleoyl-CoA ligase activity GO:0090434 IMP
Biological Process
    very long-chain fatty acid metabolic process GO:0000038 IBA
    very long-chain fatty acid metabolic process GO:0000038 IDA
    long-chain fatty acid metabolic process GO:0001676 IBA
    long-chain fatty acid metabolic process GO:0001676 IDA
    long-chain fatty acid metabolic process GO:0001676 IEA
    long-chain fatty acid metabolic process GO:0001676 ISO
    long-chain fatty acid metabolic process GO:0001676 ISS
    fatty acid metabolic process GO:0006631 IMP
    response to nutrient GO:0007584 IEP
    lipid biosynthetic process GO:0008610 IEA
    lipid biosynthetic process GO:0008610 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    positive regulation of long-chain fatty acid import across plasma membrane GO:0010747 IDA
    response to organic cyclic compound GO:0014070 IEP
    fatty acid transport GO:0015908 IGI
    fatty acid transport GO:0015908 ISO
    triglyceride biosynthetic process GO:0019432 IGI
    adiponectin-activated signaling pathway GO:0033211 ISO
    response to oleic acid GO:0034201 IEP
    long-chain fatty-acyl-CoA biosynthetic process GO:0035338 IBA
    long-chain fatty-acyl-CoA biosynthetic process GO:0035338 IGI
    xenobiotic catabolic process GO:0042178 IDA
    long-chain fatty acid import into cell GO:0044539 IMP
    long-chain fatty acid import into cell GO:0044539 ISO
    positive regulation of cold-induced thermogenesis GO:0120162 ISO
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
Subcellular Localization
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IMP
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 IEA
    peroxisomal membrane GO:0005778 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    membrane GO:0016020 IBA
 Experiment description of studies that identified Acsl1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Acsl1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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