Gene description for Mfge8
Gene name milk fat globule-EGF factor 8 protein
Gene symbol Mfge8
Other names/aliases AGS
MFGME8
MFGMP-E8
OAcGD3S
Species Rattus norvegicus
 Database cross references - Mfge8
ExoCarta ExoCarta_25277
Vesiclepedia VP_25277
Entrez Gene 25277
UniProt P70490  
 Mfge8 identified in sEVs derived from the following tissue/cell type
Dendritic cells 19812188    
Hepatocytes 19367702    
Pancreatic cancer cells 20124479    
Schwann cells 36717543    
 Gene ontology annotations for Mfge8
Molecular Function
    phosphatidylserine binding GO:0001786 ISO
    integrin binding GO:0005178 IEA
    integrin binding GO:0005178 ISO
Biological Process
    angiogenesis GO:0001525 IEA
    cell adhesion GO:0007155 IEA
    single fertilization GO:0007338 IEA
    positive regulation of cell population proliferation GO:0008284 IEP
    apoptotic cell clearance GO:0043277 IEA
    apoptotic cell clearance GO:0043277 ISO
    response to estrogen GO:0043627 IEP
Subcellular Localization
    acrosomal membrane GO:0002080 IEA
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    external side of plasma membrane GO:0009897 IEA
    external side of plasma membrane GO:0009897 ISO
 Experiment description of studies that identified Mfge8 in sEVs
1
Experiment ID 69
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19812188    
Organism Rattus norvegicus
Experiment description Immature dendritic cell-derived exosomes rescue septic animals via milk fat globule epidermal growth factor VIII.
Authors "Miksa M, Wu R, Dong W, Komura H, Amin D, Ji Y, Wang Z, Wang H, Ravikumar TS, Tracey KJ, Wang P"
Journal name JIMMU
Publication year 2009
Sample Dendritic cells
Sample name Bone marrow-derived immature dendritic cells
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 93
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
4
Experiment ID 678
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36717543    
Organism Rattus norvegicus
Experiment description Schwann cell-derived exosomes containing MFG-E8 modify macrophage/microglial polarization for attenuating inflammation via the SOCS3/STAT3 pathway after spinal cord injury
Authors "Ren J, Zhu B, Gu G, Zhang W, Li J, Wang H, Wang M, Song X, Wei Z, Feng S."
Journal name Cell Death Dis
Publication year 2023
Sample Schwann cells
Sample name Sciatic nerve-derived schwann cells
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mfge8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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