Gene description for Tst
Gene name thiosulfate sulfurtransferase
Gene symbol Tst
Other names/aliases RHODAN
Species Rattus norvegicus
 Database cross references - Tst
ExoCarta ExoCarta_25274
Vesiclepedia VP_25274
Entrez Gene 25274
UniProt P24329  
 Tst identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Tst
Molecular Function
    thiosulfate sulfurtransferase activity GO:0004792 IBA
    thiosulfate sulfurtransferase activity GO:0004792 IEA
    5S rRNA binding GO:0008097 IEA
    5S rRNA binding GO:0008097 ISO
    5S rRNA binding GO:0008097 ISS
    sulfurtransferase activity GO:0016783 TAS
    3-mercaptopyruvate sulfurtransferase activity GO:0016784 IBA
Biological Process
    iron-sulfur cluster assembly GO:0016226 NAS
    epithelial cell differentiation GO:0030855 IEA
    epithelial cell differentiation GO:0030855 ISO
    rRNA import into mitochondrion GO:0035928 IEA
    rRNA import into mitochondrion GO:0035928 ISO
    rRNA import into mitochondrion GO:0035928 ISS
    rRNA transport GO:0051029 ISO
    rRNA transport GO:0051029 ISS
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IEA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified Tst in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Tst
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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