Gene description for Gdi1
Gene name GDP dissociation inhibitor 1
Gene symbol Gdi1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gdi1
ExoCarta ExoCarta_25183
Vesiclepedia VP_25183
Entrez Gene 25183
UniProt P50398  
 Gdi1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gdi1
Molecular Function
    GDP-dissociation inhibitor activity GO:0005092 ISO
    Rab GDP-dissociation inhibitor activity GO:0005093 IBA
    Rab GDP-dissociation inhibitor activity GO:0005093 IDA
    Rab GDP-dissociation inhibitor activity GO:0005093 IEA
    GTPase activator activity GO:0005096 IEA
    small GTPase binding GO:0031267 IPI
Biological Process
    small GTPase-mediated signal transduction GO:0007264 IEA
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IBA
    Rab protein signal transduction GO:0032482 ISS
    positive regulation of toll-like receptor signaling pathway GO:0034123 ISO
    positive regulation of axon extension GO:0045773 IMP
    negative regulation of axonogenesis GO:0050771 IDA
    response to calcium ion GO:0051592 IDA
    negative regulation of protein targeting to membrane GO:0090315 ISO
    negative regulation of protein targeting to membrane GO:0090315 ISS
Subcellular Localization
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 IBA
    axon GO:0030424 IDA
    midbody GO:0030496 ISO
    protein-containing complex GO:0032991 IDA
    neuronal cell body GO:0043025 IDA
    myelin sheath GO:0043209 IDA
    presynaptic cytosol GO:0099523 IDA
 Experiment description of studies that identified Gdi1 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 101
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gdi1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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