Gene description for Cyb5r3
Gene name cytochrome b5 reductase 3
Gene symbol Cyb5r3
Other names/aliases Dia1
Nadhcb5
RNNADHCB5
Species Rattus norvegicus
 Database cross references - Cyb5r3
ExoCarta ExoCarta_25035
Vesiclepedia VP_25035
Entrez Gene 25035
UniProt P20070  
 Cyb5r3 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Cyb5r3
Molecular Function
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 IDA
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 IEA
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 ISO
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 TAS
    AMP binding GO:0016208 IDA
    oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor GO:0016653 IEA
    ADP binding GO:0043531 IDA
    nitrite reductase (NO-forming) activity GO:0050421 ISO
    flavin adenine dinucleotide binding GO:0050660 IDA
    flavin adenine dinucleotide binding GO:0050660 ISO
    NAD binding GO:0051287 IDA
    FAD binding GO:0071949 IBA
    FAD binding GO:0071949 IDA
    FAD binding GO:0071949 ISO
Biological Process
    cholesterol biosynthetic process GO:0006695 IEA
    nitric oxide biosynthetic process GO:0006809 ISO
    biological_process GO:0008150 ND
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 IEA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    lipid droplet GO:0005811 ISO
    cytosol GO:0005829 ISO
    mitochondrial membrane GO:0031966 ISO
    nitric-oxide synthase complex GO:1903958 ISO
 Experiment description of studies that identified Cyb5r3 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Cyb5r3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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