Gene description for Lamp2
Gene name lysosomal-associated membrane protein 2
Gene symbol Lamp2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Lamp2
ExoCarta ExoCarta_24944
Vesiclepedia VP_24944
Entrez Gene 24944
 Lamp2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Lamp2
Molecular Function
    protein binding GO:0005515 IPI
    ion channel inhibitor activity GO:0008200 IEA
    ion channel inhibitor activity GO:0008200 ISO
    ion channel inhibitor activity GO:0008200 ISS
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    signaling adaptor activity GO:0035591 ISO
Biological Process
    protein targeting GO:0006605 ISO
    protein targeting GO:0006605 ISS
    autophagy GO:0006914 ISO
    lysosomal lumen acidification GO:0007042 IEA
    lysosomal lumen acidification GO:0007042 ISO
    lysosomal lumen acidification GO:0007042 ISS
    biological_process GO:0008150 ND
    cellular response to starvation GO:0009267 IBA
    cellular response to starvation GO:0009267 IEA
    cellular response to starvation GO:0009267 ISO
    cellular response to starvation GO:0009267 ISS
    protein catabolic process GO:0030163 ISO
    negative regulation of protein-containing complex assembly GO:0031333 ISO
    regulation of protein stability GO:0031647 ISO
    muscle cell cellular homeostasis GO:0046716 IEA
    muscle cell cellular homeostasis GO:0046716 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    protein stabilization GO:0050821 ISS
    chaperone-mediated autophagy GO:0061684 IDA
    chaperone-mediated autophagy GO:0061684 ISO
    chaperone-mediated autophagy GO:0061684 ISO
    protein targeting to lysosome involved in chaperone-mediated autophagy GO:0061740 IBA
    protein targeting to lysosome involved in chaperone-mediated autophagy GO:0061740 IEA
    protein targeting to lysosome involved in chaperone-mediated autophagy GO:0061740 IGI
    protein targeting to lysosome involved in chaperone-mediated autophagy GO:0061740 IMP
    protein targeting to lysosome involved in chaperone-mediated autophagy GO:0061740 ISO
    protein targeting to lysosome involved in chaperone-mediated autophagy GO:0061740 ISS
    autophagosome maturation GO:0097352 IBA
    autophagosome maturation GO:0097352 IEA
    autophagosome maturation GO:0097352 ISO
    autophagosome maturation GO:0097352 ISS
    negative regulation of NLRP3 inflammasome complex assembly GO:1900226 ISO
    lysosomal protein catabolic process GO:1905146 IEA
    lysosomal protein catabolic process GO:1905146 ISO
    lysosomal protein catabolic process GO:1905146 ISS
Subcellular Localization
    autophagosome membrane GO:0000421 IBA
    autophagosome membrane GO:0000421 IEA
    autophagosome membrane GO:0000421 ISO
    extracellular space GO:0005615 ISO
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISO
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 TAS
    endosome GO:0005768 IDA
    late endosome GO:0005770 ISO
    trans-Golgi network GO:0005802 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    endosome membrane GO:0010008 IEA
    membrane GO:0016020 ISO
    phagocytic vesicle membrane GO:0030670 IEA
    phagocytic vesicle membrane GO:0030670 ISO
    platelet dense granule membrane GO:0031088 IEA
    platelet dense granule membrane GO:0031088 ISO
    late endosome membrane GO:0031902 IBA
    late endosome membrane GO:0031902 IEA
    late endosome membrane GO:0031902 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    autolysosome GO:0044754 IEA
    autolysosome GO:0044754 ISO
    membrane raft GO:0045121 IMP
    perinuclear region of cytoplasm GO:0048471 ISO
    chaperone-mediated autophagy translocation complex GO:0061742 ISO
    chaperone-mediated autophagy translocation complex GO:0061742 ISS
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    lysosomal matrix GO:1990836 IDA
 Experiment description of studies that identified Lamp2 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 99
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 100
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Lamp2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fbxo21  
Affinity Capture-MS Rattus norvegicus
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