Gene description for Gnas
Gene name GNAS complex locus
Gene symbol Gnas
Other names/aliases Gnas1
Gnpas
Nesp55
Species Rattus norvegicus
 Database cross references - Gnas
ExoCarta ExoCarta_24896
Entrez Gene 24896
UniProt P63095   Q792G6  
 Gnas identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Urine 20535238    
 Gene ontology annotations for Gnas
Molecular Function
    protein domain specific binding GO:0019904 IPI
    D1 dopamine receptor binding GO:0031748 IPI
    ionotropic glutamate receptor binding GO:0035255 IPI
    metal ion binding GO:0046872 IEA
    insulin-like growth factor receptor binding GO:0005159 IPI
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    signal transducer activity GO:0004871 ISO
    GTPase activity GO:0003924 IEA
    G-protein beta-subunit binding GO:0031681 IDA
    corticotropin-releasing hormone receptor 1 binding GO:0051430 IPI
    GTP binding GO:0005525 TAS
    G-protein alpha-subunit binding GO:0001965 IPI
    alpha-tubulin binding GO:0043014 IDA
    protein binding GO:0005515 IPI
    mu-type opioid receptor binding GO:0031852 IPI
    beta-2 adrenergic receptor binding GO:0031698 IPI
Biological Process
    skin development GO:0043588 ISO
    adenylate cyclase-activating adrenergic receptor signaling pathway GO:0071880 ISS
    positive regulation of osteoblast differentiation GO:0045669 ISO
    metabolic process GO:0008152 IEA
    calcium ion homeostasis GO:0055074 IMP
    post-embryonic body morphogenesis GO:0040032 ISO
    energy reserve metabolic process GO:0006112 ISO
    endochondral ossification GO:0001958 ISO
    negative regulation of multicellular organism growth GO:0040015 ISO
    multicellular organism growth GO:0035264 ISO
    cellular response to catecholamine stimulus GO:0071870 IDA
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 ISO
    cellular response to prostaglandin E stimulus GO:0071380 IDA
    negative regulation of renal sodium excretion GO:0035814 IMP
    G-protein coupled receptor signaling pathway GO:0007186 IMP
    skeletal system development GO:0001501 ISO
    cartilage development GO:0051216 ISO
    positive regulation of cAMP biosynthetic process GO:0030819 ISO
    platelet aggregation GO:0070527 ISO
    response to drug GO:0042493 ISO
    positive regulation of osteoclast differentiation GO:0045672 ISO
    bone development GO:0060348 ISO
    cognition GO:0050890 ISO
    embryonic hindlimb morphogenesis GO:0035116 ISO
    tissue homeostasis GO:0001894 ISO
    response to parathyroid hormone GO:0071107 ISO
    positive regulation of protein phosphorylation GO:0001934 IMP
    genetic imprinting GO:0071514 ISO
    regulation of signal transduction GO:0009966 ISO
    developmental growth GO:0048589 ISO
    adenylate cyclase-activating G-protein coupled receptor signaling pathway GO:0007189 ISO
    positive regulation of GTPase activity GO:0043547 ISS
    regulation of transcription from RNA polymerase II promoter GO:0006357 IMP
    negative regulation of blood pressure GO:0045776 IMP
    post-embryonic development GO:0009791 ISO
    hair follicle placode formation GO:0060789 ISO
    positive regulation of sodium ion transport GO:0010765 IMP
    sensory perception of chemical stimulus GO:0007606 IBA
    embryonic cranial skeleton morphogenesis GO:0048701 ISO
    DNA methylation GO:0006306 ISO
    positive regulation of cAMP-mediated signaling GO:0043950 ISS
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    dendrite GO:0030425 ISO
    ruffle GO:0001726 IDA
    vesicle GO:0031982 IDA
    synaptic vesicle GO:0008021 IEA
    cytosol GO:0005829 ISO
    membrane raft GO:0045121 IDA
    heterotrimeric G-protein complex GO:0005834 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    intrinsic component of membrane GO:0031224 ISO
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 ISO
    nucleus GO:0005634 ISO
    sarcolemma GO:0042383 IDA
    extracellular region GO:0005576 IEA
    endosome GO:0005768 IDA
    cell junction GO:0030054 IEA
    plasma membrane GO:0005886 TAS
    trans-Golgi network membrane GO:0032588 ISO
 Experiment description of studies that identified Gnas in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gnas
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gnas is involved
PathwayEvidenceSource
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome





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