Gene description for Clu
Gene name clusterin
Gene symbol Clu
Other names/aliases APOJ
CLI
DAG
RATTRPM2B
SGP-2
SGP2
SP-40
SP40
TRPM-2
TRPM2B
Trpm2
Trpmb
Species Rattus norvegicus
 Database cross references - Clu
ExoCarta ExoCarta_24854
Entrez Gene 24854
UniProt P05371  
 Clu identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Clu
Molecular Function
    ubiquitin protein ligase binding GO:0031625 ISS
    protein binding GO:0005515 IPI
    protein N-terminus binding GO:0047485 IPI
    misfolded protein binding GO:0051787 ISS
    ATPase activity GO:0016887 ISS
Biological Process
    positive regulation of tumor necrosis factor production GO:0032760 ISS
    protein import GO:0017038 ISS
    aging GO:0007568 IEP
    intrinsic apoptotic signaling pathway GO:0097193 ISS
    negative regulation of apoptotic process GO:0043066 IMP
    positive regulation of cell differentiation GO:0045597 IMP
    regulation of neuronal signal transduction GO:1902847 ISS
    spermatogenesis GO:0007283 IEA
    chaperone-mediated protein complex assembly GO:0051131 ISS
    positive regulation of neurofibrillary tangle assembly GO:1902998 ISS
    microglial cell activation GO:0001774 ISS
    cellular response to growth factor stimulus GO:0071363 IEP
    positive regulation of apoptotic process GO:0043065 ISS
    regulation of neuron death GO:1901214 ISS
    endocrine pancreas development GO:0031018 IMP
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISS
    positive regulation of tau-protein kinase activity GO:1902949 ISS
    cell morphogenesis GO:0000902 ISS
    negative regulation of beta-amyloid formation GO:1902430 ISS
    estrous cycle GO:0044849 IEP
    negative regulation of protein homooligomerization GO:0032463 ISS
    positive regulation of beta-amyloid formation GO:1902004 ISS
    positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:2000060 ISS
    response to potassium ion GO:0035864 IEP
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISS
    response to light stimulus GO:0009416 IEP
    microglial cell proliferation GO:0061518 ISS
    protein stabilization GO:0050821 ISS
    neuron projection morphogenesis GO:0048812 IMP
    chaperone-mediated protein folding GO:0061077 ISS
    response to virus GO:0009615 ISO
    positive regulation of neuron death GO:1901216 ISS
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISS
    regulation of beta-amyloid clearance GO:1900221 ISS
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902230 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISS
    response to misfolded protein GO:0051788 ISS
    central nervous system myelin maintenance GO:0032286 ISS
    positive regulation of cell proliferation GO:0008284 IMP
    response to wounding GO:0009611 IEP
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 ISO
    extracellular exosome GO:0070062 ISO
    endoplasmic reticulum GO:0005783 IEA
    mitochondrion GO:0005739 ISO
    chromaffin granule GO:0042583 IEA
    mitochondrial membrane GO:0031966 IEA
    spherical high-density lipoprotein particle GO:0034366 ISS
    intracellular GO:0005622 ISO
    nucleus GO:0005634 IDA
    neurofibrillary tangle GO:0097418 ISS
    extracellular space GO:0005615 ISS
    growth cone GO:0030426 IDA
    neuron projection GO:0043005 IDA
    cytoplasm GO:0005737 ISS
    apical dendrite GO:0097440 ISS
    aggresome GO:0016235 IDA
    extracellular matrix GO:0031012 ISS
    cytosol GO:0005829 IEA
    blood microparticle GO:0072562 ISO
 Experiment description of studies that identified Clu in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Clu
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Clu is involved
No pathways found





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