Gene description for Myh11
Gene name myosin, heavy chain 11, smooth muscle
Gene symbol Myh11
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Myh11
ExoCarta ExoCarta_24582
Vesiclepedia VP_24582
Entrez Gene 24582
 Myh11 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Myh11
Molecular Function
    microfilament motor activity GO:0000146 IBA
    cytoskeletal motor activity GO:0003774 IEA
    cytoskeletal motor activity GO:0003774 ISO
    actin binding GO:0003779 IEA
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    structural constituent of muscle GO:0008307 IEA
    structural constituent of muscle GO:0008307 ISO
    structural constituent of muscle GO:0008307 NAS
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IEA
Biological Process
    smooth muscle contraction GO:0006939 ISO
    actin filament organization GO:0007015 IBA
    elastic fiber assembly GO:0048251 IEA
    elastic fiber assembly GO:0048251 ISO
    cardiac muscle cell development GO:0055013 IEA
    cardiac muscle cell development GO:0055013 ISO
Subcellular Localization
    stress fiber GO:0001725 ISO
    cytoplasm GO:0005737 IBA
    muscle myosin complex GO:0005859 ISO
    muscle myosin complex GO:0005859 NAS
    brush border GO:0005903 ISO
    actin cytoskeleton GO:0015629 IBA
    membrane GO:0016020 IBA
    myosin complex GO:0016459 IEA
    myosin complex GO:0016459 ISO
    myofibril GO:0030016 IEA
    smooth muscle contractile fiber GO:0030485 ISO
    myosin filament GO:0032982 IEA
    melanosome GO:0042470 IEA
 Experiment description of studies that identified Myh11 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Myh11
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Myh11 is involved
No pathways found





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