Gene ontology annotations for Gapdh
Experiment description of studies that identified Gapdh in exosomes
1
Experiment ID
225
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25998041
Organism
Rattus norvegicus
Experiment description
Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors
Lee JE, Moon PG, Lee IK, Baek MC
Journal name
Protein J
Publication year
2015
Sample
Adipocytes
Sample name
Adipocytes
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
2
Experiment ID
30
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
FLOT
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
16446100
Organism
Rattus norvegicus
Experiment description
Exosomes are released by cultured cortical neurones.
Authors
Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R
Journal name
MCN
Publication year
2006
Sample
Cortical neurones
Sample name
Cortical neurones
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.13 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF] Western blotting
3
Experiment ID
35
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD81|CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19367702
Organism
Rattus norvegicus
Experiment description
Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors
Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name
JPR
Publication year
2008
Sample
Hepatocytes
Sample name
Hepatocytes
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF] Western blotting
4
Experiment ID
92
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|GAPDH
EV Cytosolic markers
✔
CD9|CD151
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [MALDI TOF]
PubMed ID
20124479
Organism
Rattus norvegicus
Experiment description
Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors
Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name
CR
Publication year
2010
Sample
Pancreatic cancer cells
Sample name
AS
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.14-1.17 g/mL
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-PCR Western blotting RatRef-12 Microarray FACS Mass spectrometry [MALDI TOF]
5
Experiment ID
90
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70|HSP90|TSG101
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [Orbitrap]
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D2 Rep 1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.16-1.21 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
6
Experiment ID
94
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [Orbitrap]
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D4 Rep 1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.16-1.21 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
7
Experiment ID
95
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [Orbitrap]
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D7 Rep 1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.25 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
8
Experiment ID
96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [Orbitrap]
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D2 Rep 2
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.16-1.21 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
9
Experiment ID
97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [Orbitrap]
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D4 Rep 2
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.16-1.21 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
10
Experiment ID
98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [Orbitrap]
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D7 Rep 2
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.25 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
11
Experiment ID
99
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [QSTAR]
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D2 Rep 3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.16-1.21 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
12
Experiment ID
100
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [QSTAR]
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D4 Rep 3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.16-1.21 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
13
Experiment ID
101
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [QSTAR]
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D7 Rep 3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.25 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
14
Experiment ID
192
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
CD63|CD81|FLOT
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20535238
Organism
Rattus norvegicus
Experiment description
Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors
Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name
Proteomics Clin Appl
Publication year
2010
Sample
Urine
Sample name
Urine - Rats of liver injury
Isolation/purification methods
Differential centrifugation Filtration UltracentrifugationSucrose cushion Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for Gapdh
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
Ncald
Affinity Capture-MS
Rattus norvegicus
Affinity Capture-Western
Rattus norvegicus
View the network
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Pathways in which Gapdh is involved