Experiment description of studies that identified Calm1 in sEVs

1 Experiment ID 35 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|CD81|CD63 Enriched markers ✔ HSPA5 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19367702     Organism Rattus norvegicus Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes. Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM" Journal name JPR Publication year 2008 Sample Hepatocytes Sample name Hepatocytes Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting

2 Experiment ID 90 MISEV standards ✔ EM Biophysical techniques ✔ HSC70|HSP90|TSG101|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E" Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

3 Experiment ID 96 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP90|HSC70|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

4 Experiment ID 99 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP90|HSC70|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [QSTAR] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

5 Experiment ID 100 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP90|HSC70|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [QSTAR] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

6 Experiment ID 192 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|HSP70|CD63|CD81|FLOT Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20535238     Organism Rattus norvegicus Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples. Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M." Journal name Proteomics Clin Appl Publication year 2010 Sample Urine Sample name Urine - Rats of liver injury Isolation/purification methods Differential centrifugation Filtration UltracentrifugationSucrose cushion Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for Calm1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Vipr2   Reconstituted Complex Rattus norvegicus 2 Gja1   Affinity Capture-MS Rattus norvegicus 3 Scn3a   Reconstituted Complex Rattus norvegicus 4 NRGN   Two-hybrid Homo sapiens 5 Glp1r   Reconstituted Complex Rattus norvegicus 6 Glp2r   Reconstituted Complex Rattus norvegicus 7 Scn8a   Reconstituted Complex Mus musculus 8 Nrgn   Co-purification Rattus norvegicus 9 Adora2a   Affinity Capture-MS Rattus norvegicus PCA Rattus norvegicus 10 Crhr1   Reconstituted Complex Rattus norvegicus 11 Scn4a   Reconstituted Complex Rattus norvegicus Affinity Capture-Western Rattus norvegicus 12 Vipr1   Reconstituted Complex Rattus norvegicus 13 Gad1   Affinity Capture-Western Rattus norvegicus 14 Pth1r   Reconstituted Complex Rattus norvegicus 15 Grm7   Reconstituted Complex Rattus norvegicus 16 Snca   Affinity Capture-MS Rattus norvegicus 17 NOS3   Co-crystal Structure Homo sapiens 18 Pth2r   Reconstituted Complex Rattus norvegicus 19 Gad2   Affinity Capture-Western Rattus norvegicus 20 Calcr   Reconstituted Complex Rattus norvegicus 21 Sctr   Reconstituted Complex Rattus norvegicus 22 Itm2b 290364 Affinity Capture-MS Rattus norvegicus 23 SCN1A   Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens 24 Drd2   PCA Rattus norvegicus 25 Scn2a   Reconstituted Complex Rattus norvegicus View the network

Pathways in which Calm1 is involved

No pathways found