Gene description for C4a
Gene name complement component 4A (Rodgers blood group)
Gene symbol C4a
Other names/aliases C4
C4-1
C4b
Species Rattus norvegicus
 Database cross references - C4a
ExoCarta ExoCarta_24233
Vesiclepedia VP_24233
Entrez Gene 24233
UniProt P08649  
 C4a identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for C4a
Molecular Function
    complement component C1q complex binding GO:0001849 ISO
    endopeptidase inhibitor activity GO:0004866 IEA
Biological Process
    inflammatory response GO:0006954 IEA
    complement activation GO:0006956 IBA
    complement activation GO:0006956 ISO
    complement activation GO:0006956 ISO
    complement activation GO:0006956 TAS
    complement activation, classical pathway GO:0006958 IEA
    innate immune response GO:0045087 IEA
    positive regulation of apoptotic cell clearance GO:2000427 ISO
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    axon GO:0030424 IEA
    axon GO:0030424 ISO
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    neuronal cell body GO:0043025 ISO
    synapse GO:0045202 IEA
    synapse GO:0045202 ISO
 Experiment description of studies that identified C4a in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for C4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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