Gene description for Dip2b
Gene name
DIP2 disco-interacting protein 2 homolog B (Drosophila)
Gene symbol
Dip2b
Other names/aliases
4932422C22 AI317237 AI854602 mKIAA1463
Species
Mus musculus
Database cross references - Dip2b
ExoCarta
ExoCarta_239667
Vesiclepedia
VP_239667
Entrez Gene
239667
UniProt
Q3UH60
Dip2b identified in sEVs derived from the following tissue/cell type
Colon cancer cells
37309723
Macrophages
23658846
Macrophages
23658846
Macrophages
23658846
T-cell lymphoma cells
37309723
Gene ontology annotations for Dip2b
Experiment description of studies that identified Dip2b in sEVs
1
Experiment ID
907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
37309723
Organism
Mus musculus
Experiment description
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name
Proteomics
Publication year
2023
Sample
Colon cancer cells
Sample name
C26
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
2
Experiment ID
214
MISEV standards
✔
EM
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
"Hassani K, Olivier M."
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
3
Experiment ID
215
MISEV standards
✔
EM
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
"Hassani K, Olivier M."
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
LPS-treated-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
216
MISEV standards
✔
EM
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
"Hassani K, Olivier M."
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
Normal-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
5
Experiment ID
908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
37309723
Organism
Mus musculus
Experiment description
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name
Proteomics
Publication year
2023
Sample
T-cell lymphoma cells
Sample name
EL4
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for Dip2b
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
Zbtb7b
Affinity Capture-MS
Mus musculus
2
Fmr1
Affinity Capture-MS
Mus musculus
3
Pde4dip
Affinity Capture-MS
Mus musculus
4
Dlg4
Affinity Capture-MS
Mus musculus
Affinity Capture-MS
Mus musculus
5
Atg16l1
Affinity Capture-MS
Mus musculus
6
Micu1
Co-fractionation
Mus musculus
View the network
image/svg+xml
Pathways in which Dip2b is involved
No pathways found