Gene description for Nt5e
Gene name 5' nucleotidase, ecto
Gene symbol Nt5e
Other names/aliases 2210401F01Rik
AI447961
CD73
NT
Nt5
eNT
Species Mus musculus
 Database cross references - Nt5e
ExoCarta ExoCarta_23959
Vesiclepedia VP_23959
Entrez Gene 23959
UniProt Q61503  
 Nt5e identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nt5e
Molecular Function
    nucleotide binding GO:0000166 IEA
    5'-deoxynucleotidase activity GO:0002953 IEA
    5'-deoxynucleotidase activity GO:0002953 ISO
    ferrous iron binding GO:0008198 ISO
    5'-nucleotidase activity GO:0008253 IBA
    5'-nucleotidase activity GO:0008253 IDA
    5'-nucleotidase activity GO:0008253 IMP
    5'-nucleotidase activity GO:0008253 ISO
    zinc ion binding GO:0008270 IEA
    zinc ion binding GO:0008270 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    thymidylate 5'-phosphatase activity GO:0050340 IEA
    thymidylate 5'-phosphatase activity GO:0050340 ISO
    IMP 5'-nucleotidase activity GO:0050483 IEA
    IMP 5'-nucleotidase activity GO:0050483 ISO
    GMP 5'-nucleotidase activity GO:0050484 IEA
    GMP 5'-nucleotidase activity GO:0050484 ISO
    XMP 5'-nucleosidase activity GO:0106411 IEA
Biological Process
    AMP catabolic process GO:0006196 IBA
    AMP catabolic process GO:0006196 IMP
    AMP catabolic process GO:0006196 IMP
    AMP catabolic process GO:0006196 ISO
    leukocyte cell-cell adhesion GO:0007159 IEA
    leukocyte cell-cell adhesion GO:0007159 ISO
    response to ATP GO:0033198 IEA
    ADP catabolic process GO:0046032 IMP
    ATP metabolic process GO:0046034 IEA
    adenosine metabolic process GO:0046085 ISO
    adenosine biosynthetic process GO:0046086 IMP
    adenosine biosynthetic process GO:0046086 ISO
    negative regulation of inflammatory response GO:0050728 IMP
    calcium ion homeostasis GO:0055074 IGI
    inhibition of non-skeletal tissue mineralization GO:0140928 IMP
    inhibition of non-skeletal tissue mineralization GO:0140928 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    external side of plasma membrane GO:0009897 IMP
    cell surface GO:0009986 ISO
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    synaptic membrane GO:0097060 ISO
 Experiment description of studies that identified Nt5e in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nt5e
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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