Gene description for SLC7A8
Gene name solute carrier family 7 (amino acid transporter light chain, L system), member 8
Gene symbol SLC7A8
Other names/aliases LAT2
LPI-PC1
Species Homo sapiens
 Database cross references - SLC7A8
ExoCarta ExoCarta_23428
Vesiclepedia VP_23428
Entrez Gene 23428
HGNC 11066
MIM 604235
UniProt Q9UHI5  
 SLC7A8 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SLC7A8
Molecular Function
    protein binding GO:0005515 IPI
    organic cation transmembrane transporter activity GO:0015101 IDA
    amino acid transmembrane transporter activity GO:0015171 EXP
    amino acid transmembrane transporter activity GO:0015171 IDA
    amino acid transmembrane transporter activity GO:0015171 ISS
    neutral L-amino acid transmembrane transporter activity GO:0015175 IBA
    neutral L-amino acid transmembrane transporter activity GO:0015175 IDA
    neutral L-amino acid transmembrane transporter activity GO:0015175 IMP
    neutral L-amino acid transmembrane transporter activity GO:0015175 ISS
    L-amino acid transmembrane transporter activity GO:0015179 IBA
    L-alanine transmembrane transporter activity GO:0015180 IGI
    glycine transmembrane transporter activity GO:0015187 ISS
    L-leucine transmembrane transporter activity GO:0015190 IDA
    L-leucine transmembrane transporter activity GO:0015190 IGI
    antiporter activity GO:0015297 IDA
    thyroid hormone transmembrane transporter activity GO:0015349 IDA
    thyroid hormone transmembrane transporter activity GO:0015349 ISS
    toxin transmembrane transporter activity GO:0019534 IDA
    peptide antigen binding GO:0042605 ISS
    protein heterodimerization activity GO:0046982 IDA
Biological Process
    amino acid transmembrane transport GO:0003333 IBA
    amino acid transport GO:0006865 IDA
    amino acid transport GO:0006865 TAS
    neutral amino acid transport GO:0015804 IBA
    neutral amino acid transport GO:0015804 IDA
    neutral amino acid transport GO:0015804 ISS
    glycine transport GO:0015816 ISS
    L-leucine transport GO:0015820 ISS
    tryptophan transport GO:0015827 ISS
    valine transport GO:0015829 ISS
    proline transmembrane transport GO:0035524 ISS
    thyroid hormone transport GO:0070327 IDA
    thyroid hormone transport GO:0070327 ISS
    amino acid import across plasma membrane GO:0089718 IDA
    transport across blood-brain barrier GO:0150104 NAS
    L-leucine import across plasma membrane GO:1903801 IDA
    L-leucine import across plasma membrane GO:1903801 IGI
    L-leucine import across plasma membrane GO:1903801 IMP
    L-alanine import across plasma membrane GO:1904273 IGI
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IGI
    plasma membrane GO:0005886 TAS
    basal plasma membrane GO:0009925 IDA
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 ISS
    microvillus membrane GO:0031528 IDA
 Experiment description of studies that identified SLC7A8 in sEVs
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC7A8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PXMP2  
Affinity Capture-MS Homo sapiens
2 FLVCR1 28982
Affinity Capture-MS Homo sapiens
3 FITM2  
Affinity Capture-MS Homo sapiens
4 SLC3A2 6520
Affinity Capture-MS Homo sapiens
5 SLC3A1 6519
Affinity Capture-Western Homo sapiens
6 GPR89A  
Affinity Capture-MS Homo sapiens
7 FADS1 3992
Affinity Capture-MS Homo sapiens
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