Experiment description of studies that identified Gprc5a in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 303 MISEV standards ✔ EM Biophysical techniques ✔ Cd63|Cd81|Cd9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33431899     Organism Mus musculus Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." Journal name Nat Commun Publication year 2021 Sample Faeces Sample name Intestinal epithelial cells - C57BL/6 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient centrifugation Flotation density - Molecules identified in the study Protein Lipid miRNA Methods used in the study Western blotting Mass spectrometry Immunofluorescence miRNA array HPLC

3 Experiment ID 304 MISEV standards ✔ EM Biophysical techniques ✔ Cd63|Cd81|Cd9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33431899     Organism Mus musculus Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." Journal name Nat Commun Publication year 2021 Sample Faeces Sample name Intestinal epithelial cells - C57BL/6 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient centrifugation Flotation density - Molecules identified in the study Protein Lipid miRNA Methods used in the study Western blotting Mass spectrometry Immunofluorescence miRNA array HPLC

4 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Gprc5a

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Gprc5a is involved

No pathways found