Gene description for Wnk1
Gene name WNK lysine deficient protein kinase 1
Gene symbol Wnk1
Other names/aliases 6430573H23Rik
EG406236
Hsn2
Prkwnk1
mKIAA0344
Species Mus musculus
 Database cross references - Wnk1
ExoCarta ExoCarta_232341
Entrez Gene 232341
UniProt P83741  
 Wnk1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wnk1
Molecular Function
    protein kinase inhibitor activity GO:0004860 IEA
    ATP binding GO:0005524 ISO
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activity GO:0004674 ISO
    magnesium ion binding GO:0000287 ISO
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    potassium channel inhibitor activity GO:0019870 ISO
    kinase activity GO:0016301 IEA
    protein kinase binding GO:0019901 ISO
    transferase activity GO:0016740 IEA
    protein serine/threonine kinase inhibitor activity GO:0030291 ISO
    protein kinase activity GO:0004672 IDA
    nucleotide binding GO:0000166 IEA
    chloride channel inhibitor activity GO:0019869 ISO
    phosphatase binding GO:0019902 ISO
Biological Process
    negative regulation of kinase activity GO:0033673 ISO
    protein autophosphorylation GO:0046777 ISO
    protein phosphorylation GO:0006468 ISO
    negative regulation of protein serine/threonine kinase activity GO:0071901 ISO
    positive regulation of systemic arterial blood pressure GO:0003084 IMP
    negative regulation of phosphatase activity GO:0010923 ISO
    intracellular signal transduction GO:0035556 ISO
    regulation of cellular process GO:0050794 IDA
    negative regulation of pancreatic juice secretion GO:0090188 IMP
    ion transport GO:0006811 IDA
    phosphorylation GO:0016310 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Wnk1 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wnk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Wnk1 is involved
PathwayEvidenceSource
Stimuli-sensing channels IEA Reactome





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