Gene description for Ikbkap
Gene name inhibitor of kappa light polypeptide enhancer in B cells, kinase complex-associated protein
Gene symbol Ikbkap
Other names/aliases 3110040G09Rik
6030413P05
C78473
Elp1
IKAP
Species Mus musculus
 Database cross references - Ikbkap
ExoCarta ExoCarta_230233
Vesiclepedia VP_230233
Entrez Gene 230233
UniProt Q7TT37  
 Ikbkap identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ikbkap
Molecular Function
    tRNA binding GO:0000049 IBA
    protein kinase activity GO:0004672 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 ISO
Biological Process
    tRNA wobble uridine modification GO:0002098 IMP
    tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation GO:0002926 IBA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    elongator holoenzyme complex GO:0033588 IBA
    elongator holoenzyme complex GO:0033588 ISO
    elongator holoenzyme complex GO:0033588 ISS
 Experiment description of studies that identified Ikbkap in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ikbkap
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Atg16l1  
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Chaf1a  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ikbkap is involved
No pathways found





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