Gene description for Aldob
Gene name aldolase B, fructose-bisphosphate
Gene symbol Aldob
Other names/aliases Aldo-2
Aldo2
BC016435
Species Mus musculus
 Database cross references - Aldob
ExoCarta ExoCarta_230163
Vesiclepedia VP_230163
Entrez Gene 230163
UniProt Q91Y97  
 Aldob identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Aldob
Molecular Function
    fructose-bisphosphate aldolase activity GO:0004332 IBA
    fructose-bisphosphate aldolase activity GO:0004332 IDA
    fructose-bisphosphate aldolase activity GO:0004332 IMP
    fructose-bisphosphate aldolase activity GO:0004332 ISO
    cytoskeletal protein binding GO:0008092 IEA
    cytoskeletal protein binding GO:0008092 ISO
    phosphatidylcholine binding GO:0031210 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    ATPase binding GO:0051117 IEA
    ATPase binding GO:0051117 ISO
    molecular adaptor activity GO:0060090 IEA
    molecular adaptor activity GO:0060090 ISO
    fructose-1-phosphate aldolase activity GO:0061609 IBA
    fructose-1-phosphate aldolase activity GO:0061609 IMP
    fructose-1-phosphate aldolase activity GO:0061609 ISO
    fructose-1-phosphate aldolase activity GO:0061609 ISS
    fructose binding GO:0070061 IEA
    fructose binding GO:0070061 ISO
Biological Process
    fructose metabolic process GO:0006000 ISO
    fructose catabolic process GO:0006001 IDA
    fructose catabolic process GO:0006001 IMP
    gluconeogenesis GO:0006094 IEA
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 ISO
    glycolytic process GO:0006096 ISS
    NADH oxidation GO:0006116 IEA
    NADH oxidation GO:0006116 ISO
    response to zinc ion GO:0010043 ISO
    fructose 1,6-bisphosphate metabolic process GO:0030388 IBA
    fructose 1,6-bisphosphate metabolic process GO:0030388 IEA
    fructose 1,6-bisphosphate metabolic process GO:0030388 ISO
    positive regulation of ATP-dependent activity GO:0032781 ISO
    response to peptide hormone GO:0043434 ISO
    glycolytic process through fructose-6-phosphate GO:0061615 IC
    fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate GO:0061624 IC
    fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate GO:0061624 IMP
    glycolytic process through fructose-1-phosphate GO:0061625 IC
    vacuolar proton-transporting V-type ATPase complex assembly GO:0070072 IEA
    vacuolar proton-transporting V-type ATPase complex assembly GO:0070072 ISO
    negative regulation of pentose-phosphate shunt GO:1905856 IEA
    negative regulation of pentose-phosphate shunt GO:1905856 ISO
Subcellular Localization
    microtubule organizing center GO:0005815 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    rough endoplasmic reticulum membrane GO:0030867 ISO
    smooth endoplasmic reticulum membrane GO:0030868 ISO
    centriolar satellite GO:0034451 IEA
    centriolar satellite GO:0034451 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Aldob in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Aldob
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Bbs2  
Co-fractionation Mus musculus
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