Gene description for Mfsd7b
Gene name major facilitator superfamily domain containing 7B
Gene symbol Mfsd7b
Other names/aliases 9630055N22Rik
FLVCR
Flvcr1
Species Mus musculus
 Database cross references - Mfsd7b
ExoCarta ExoCarta_226844
Vesiclepedia VP_226844
Entrez Gene 226844
UniProt B2RXV4  
 Mfsd7b identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Mfsd7b
Molecular Function
    choline transmembrane transporter activity GO:0015220 ISS
    heme transmembrane transporter activity GO:0015232 IBA
    heme transmembrane transporter activity GO:0015232 IEA
    heme transmembrane transporter activity GO:0015232 ISO
    heme binding GO:0020037 IBA
    ethanolamine transmembrane transporter activity GO:0034228 ISS
Biological Process
    blood vessel development GO:0001568 IMP
    in utero embryonic development GO:0001701 IMP
    mitochondrial transport GO:0006839 IBA
    mitochondrial transport GO:0006839 ISO
    phospholipid biosynthetic process GO:0008654 ISS
    choline transport GO:0015871 IEA
    heme transport GO:0015886 IEA
    heme transport GO:0015886 ISO
    erythrocyte differentiation GO:0030218 IBA
    erythrocyte differentiation GO:0030218 IMP
    erythrocyte differentiation GO:0030218 ISO
    ethanolamine transport GO:0034229 IEA
    limb morphogenesis GO:0035108 IMP
    multicellular organism growth GO:0035264 IMP
    embryonic digit morphogenesis GO:0042733 IMP
    erythrocyte maturation GO:0043249 IEA
    regulation of organ growth GO:0046620 IMP
    spleen development GO:0048536 IMP
    embryonic skeletal system morphogenesis GO:0048704 IMP
    transmembrane transport GO:0055085 IEA
    head morphogenesis GO:0060323 IMP
    heme export GO:0097037 IBA
    heme export GO:0097037 ISO
    heme export GO:0097037 ISS
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 IBA
    mitochondrial membrane GO:0031966 IDA
    mitochondrial membrane GO:0031966 IEA
 Experiment description of studies that identified Mfsd7b in sEVs
1
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Mfsd7b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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