Gene description for Fam49b
Gene name family with sequence similarity 49, member B
Gene symbol Fam49b
Other names/aliases 0910001A06Rik
AW122079
Species Mus musculus
 Database cross references - Fam49b
ExoCarta ExoCarta_223601
Vesiclepedia VP_223601
Entrez Gene 223601
UniProt Q921M7  
 Fam49b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Fam49b
Molecular Function
    protein binding GO:0005515 IPI
    MHC class Ib protein binding, via antigen binding groove GO:0023030 IDA
    small GTPase binding GO:0031267 IEA
    small GTPase binding GO:0031267 ISO
Biological Process
    positive regulation of T cell mediated cytotoxicity GO:0001916 IDA
    regulation of cell migration GO:0030334 ISO
    regulation of cell migration GO:0030334 ISS
    negative regulation of actin filament polymerization GO:0030837 ISO
    negative regulation of actin filament polymerization GO:0030837 ISS
    positive regulation of type II interferon production GO:0032729 IDA
    positive regulation of T cell activation GO:0050870 IBA
    positive regulation of T cell activation GO:0050870 IDA
    regulation of chemotaxis GO:0050920 ISO
    regulation of chemotaxis GO:0050920 ISS
    negative regulation of small GTPase mediated signal transduction GO:0051058 IMP
    negative regulation of small GTPase mediated signal transduction GO:0051058 ISO
    negative regulation of small GTPase mediated signal transduction GO:0051058 ISS
    cellular response to molecule of bacterial origin GO:0071219 IMP
    cellular response to molecule of bacterial origin GO:0071219 ISO
    cellular response to molecule of bacterial origin GO:0071219 ISS
    regulation of mitochondrial fission GO:0090140 IMP
    regulation of mitochondrial fission GO:0090140 ISO
    regulation of mitochondrial fission GO:0090140 ISS
    regulation of establishment of cell polarity GO:2000114 ISO
    regulation of establishment of cell polarity GO:2000114 ISS
    positive regulation of memory T cell activation GO:2000568 IDA
Subcellular Localization
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    cilium GO:0005929 IDA
    membrane GO:0016020 IEA
 Experiment description of studies that identified Fam49b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Fam49b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Tsc1  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Unk  
Affinity Capture-RNA Mus musculus
View the network image/svg+xml



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