Gene description for Usp4
Gene name ubiquitin specific peptidase 4 (proto-oncogene)
Gene symbol Usp4
Other names/aliases F730026I20Rik
Unp
mKIAA4155
Species Mus musculus
 Database cross references - Usp4
ExoCarta ExoCarta_22258
Vesiclepedia VP_22258
Entrez Gene 22258
UniProt P35123  
 Usp4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Usp4
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 ISO
    cysteine-type deubiquitinase activity GO:0004843 ISS
    protein binding GO:0005515 IPI
    adenosine receptor binding GO:0031685 IEA
    adenosine receptor binding GO:0031685 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    spliceosomal tri-snRNP complex assembly GO:0000244 ISO
    spliceosomal tri-snRNP complex assembly GO:0000244 ISS
    proteolysis GO:0006508 IEA
    protein deubiquitination GO:0016579 ISO
    protein deubiquitination GO:0016579 ISS
    negative regulation of protein ubiquitination GO:0031397 ISO
    negative regulation of protein ubiquitination GO:0031397 ISS
    regulation of protein stability GO:0031647 ISO
    regulation of protein stability GO:0031647 ISS
    protein localization to cell surface GO:0034394 ISO
    protein localization to cell surface GO:0034394 ISS
    positive regulation of TORC1 signaling GO:1904263 ISO
    positive regulation of TORC1 signaling GO:1904263 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Usp4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Usp4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Ubc  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
View the network image/svg+xml



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