Gene description for Tuba3b
Gene name tubulin, alpha 3B
Gene symbol Tuba3b
Other names/aliases M[a]7
Tuba7
Species Mus musculus
 Database cross references - Tuba3b
ExoCarta ExoCarta_22147
Vesiclepedia VP_22147
Entrez Gene 22147
UniProt P05214  
 Tuba3b identified in sEVs derived from the following tissue/cell type
Fibroblasts 23260141    
Oligodendrocytes 21136642    
 Gene ontology annotations for Tuba3b
Molecular Function
    molecular_function GO:0003674 ND
Biological Process
    male germ-line stem cell population maintenance GO:0036098 IGI
Subcellular Localization
    microtubule cytoskeleton GO:0015630 ISO
 Experiment description of studies that identified Tuba3b in sEVs
1
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Tuba3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
2 Usp7  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Tuba3b is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Aggrephagy IEA Reactome
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Asparagine N-linked glycosylation IEA Reactome
Autophagy IEA Reactome
Axon guidance IEA Reactome
Carboxyterminal post-translational modifications of tubulin IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Cilium Assembly IEA Reactome
COPI-dependent Golgi-to-ER retrograde traffic IEA Reactome
COPI-independent Golgi-to-ER retrograde traffic IEA Reactome
COPI-mediated anterograde transport IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Developmental Biology IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ER to Golgi Anterograde Transport IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
G2/M Transition IEA Reactome
Gap junction assembly IEA Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking and regulation IEA Reactome
Golgi-to-ER retrograde transport IEA Reactome
Hedgehog 'off' state IEA Reactome
Hemostasis IEA Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand IEA Reactome
Immune System IEA Reactome
Interferon Signaling IEA Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic IEA Reactome
Intraflagellar transport IEA Reactome
Kinesins IEA Reactome
L1CAM interactions IEA Reactome
M Phase IEA Reactome
Macroautophagy IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
MHC class II antigen presentation IEA Reactome
Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Nervous system development IEA Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
PKR-mediated signaling IEA Reactome
Post-translational protein modification IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Recycling pathway of L1 IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
Sealing of the nuclear envelope (NE) by ESCRT-III IEA Reactome
Selective autophagy IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint IEA Reactome
Transport of connexons to the plasma membrane IEA Reactome
Transport to the Golgi and subsequent modification IEA Reactome
Vesicle-mediated transport IEA Reactome





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