Gene description for Timp1
Gene name tissue inhibitor of metalloproteinase 1
Gene symbol Timp1
Other names/aliases Clgi
EPA
TIMP-1
TPA-S1
Timp
Species Mus musculus
 Database cross references - Timp1
ExoCarta ExoCarta_21857
Vesiclepedia VP_21857
Entrez Gene 21857
UniProt P12032  
 Timp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Timp1
Molecular Function
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    cytokine activity GO:0005125 ISO
    cytokine activity GO:0005125 ISS
    growth factor activity GO:0008083 IEA
    metalloendopeptidase inhibitor activity GO:0008191 IBA
    metalloendopeptidase inhibitor activity GO:0008191 ISO
    metalloendopeptidase inhibitor activity GO:0008191 ISS
    zinc ion binding GO:0008270 IEA
    zinc ion binding GO:0008270 ISO
    peptidase inhibitor activity GO:0030414 ISO
Biological Process
    connective tissue replacement involved in inflammatory response wound healing GO:0002248 IDA
    signal transduction GO:0007165 IEA
    positive regulation of cell population proliferation GO:0008284 ISO
    positive regulation of cell population proliferation GO:0008284 ISS
    response to hormone GO:0009725 IBA
    negative regulation of endopeptidase activity GO:0010951 ISO
    negative regulation of endopeptidase activity GO:0010951 ISS
    response to cytokine GO:0034097 IBA
    response to cytokine GO:0034097 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of catalytic activity GO:0043086 ISO
    negative regulation of catalytic activity GO:0043086 ISS
    response to peptide hormone GO:0043434 IEA
    negative regulation of membrane protein ectodomain proteolysis GO:0051045 IBA
    negative regulation of membrane protein ectodomain proteolysis GO:0051045 IEA
    negative regulation of membrane protein ectodomain proteolysis GO:0051045 ISO
    cartilage development GO:0051216 IEA
    cellular response to UV-A GO:0071492 ISO
    cellular response to UV-A GO:0071492 ISS
    negative regulation of trophoblast cell migration GO:1901164 IEA
    negative regulation of trophoblast cell migration GO:1901164 ISO
    cellular response to peptide GO:1901653 IEA
    negative regulation of metallopeptidase activity GO:1905049 ISO
    regulation of integrin-mediated signaling pathway GO:2001044 ISO
    regulation of integrin-mediated signaling pathway GO:2001044 ISS
Subcellular Localization
    basement membrane GO:0005604 IDA
    basement membrane GO:0005604 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    extracellular matrix GO:0031012 IBA
    extracellular matrix GO:0031012 IDA
 Experiment description of studies that identified Timp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Timp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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