Gene description for Thbs4
Gene name thrombospondin 4
Gene symbol Thbs4
Other names/aliases TSP4
Species Mus musculus
 Database cross references - Thbs4
ExoCarta ExoCarta_21828
Vesiclepedia VP_21828
Entrez Gene 21828
UniProt Q9Z1T2  
 Thbs4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Thbs4
Molecular Function
    fibronectin binding GO:0001968 ISO
    integrin binding GO:0005178 IEA
    integrin binding GO:0005178 ISO
    extracellular matrix structural constituent GO:0005201 RCA
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 ISO
    growth factor activity GO:0008083 IEA
    heparin binding GO:0008201 IEA
    heparin binding GO:0008201 ISO
    identical protein binding GO:0042802 ISO
    laminin-1 binding GO:0043237 ISO
Biological Process
    positive regulation of endothelial cell proliferation GO:0001938 IEA
    positive regulation of endothelial cell proliferation GO:0001938 ISO
    response to unfolded protein GO:0006986 IEA
    signal transduction GO:0007165 IEA
    nervous system development GO:0007399 ISO
    negative regulation of angiogenesis GO:0016525 IEA
    regulation of tissue remodeling GO:0034103 IMP
    response to endoplasmic reticulum stress GO:0034976 IBA
    response to endoplasmic reticulum stress GO:0034976 IMP
    behavioral response to pain GO:0048266 IMP
    behavioral response to pain GO:0048266 ISO
    tissue remodeling GO:0048771 IEA
    neuron projection morphogenesis GO:0048812 ISO
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    myoblast migration GO:0051451 IEA
    myoblast migration GO:0051451 ISO
    positive regulation of cell division GO:0051781 IEA
    endothelial cell-cell adhesion GO:0071603 IEA
    endothelial cell-cell adhesion GO:0071603 ISO
    positive regulation of neutrophil chemotaxis GO:0090023 IEA
    positive regulation of neutrophil chemotaxis GO:0090023 ISO
Subcellular Localization
    basement membrane GO:0005604 IEA
    basement membrane GO:0005604 ISO
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    endoplasmic reticulum GO:0005783 IDA
    sarcoplasmic reticulum GO:0016529 IDA
    neuromuscular junction GO:0031594 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 IDA
 Experiment description of studies that identified Thbs4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Thbs4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Thbs4 is involved
PathwayEvidenceSource
Signal Transduction IEA Reactome
Signaling by PDGF IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome





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