Experiment description of studies that identified Psmd2 in exosomes |
1 |
Experiment ID |
210 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD81|FLOT1
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23260141
|
Organism |
Mus musculus |
Experiment description |
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. |
Authors |
Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL. |
Journal name |
Cell
|
Publication year |
2012 |
Sample |
Fibroblasts |
Sample name |
Normal-Fibroblasts (L cells) |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
2 |
Experiment ID |
214 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
Leishmania-infected-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
3 |
Experiment ID |
215 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
LPS-treated-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
4 |
Experiment ID |
216 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
Normal-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
5 |
Experiment ID |
15 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
17486113
|
Organism |
Mus musculus Homo sapiens |
Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
Authors |
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO |
Journal name |
NCB
|
Publication year |
2007 |
Sample |
Mast cells |
Sample name |
MC9 Bone marrow-derived mast cells HMC-1 |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.11-1.21 g/mL
|
Molecules identified in the study |
Protein mRNA miRNA |
Methods used in the study |
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array |
|
|
6 |
Experiment ID |
264 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63|CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25242146
|
Organism |
Mus musculus |
Experiment description |
Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells |
Authors |
Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino |
Journal name |
Molecular Cell
|
Publication year |
2014 |
Sample |
Neural stem cells |
Sample name |
NPCs - Th2 treated |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.20 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|