Gene description for Rnpep
Gene name arginyl aminopeptidase (aminopeptidase B)
Gene symbol Rnpep
Other names/aliases -
Species Mus musculus
 Database cross references - Rnpep
ExoCarta ExoCarta_215615
Vesiclepedia VP_215615
Entrez Gene 215615
UniProt Q8VCT3  
 Rnpep identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rnpep
Molecular Function
    aminopeptidase activity GO:0004177 ISO
    aminopeptidase activity GO:0004177 ISS
    copper ion binding GO:0005507 ISO
    metallopeptidase activity GO:0008237 IEA
    zinc ion binding GO:0008270 IEA
    zinc ion binding GO:0008270 ISO
    peptide binding GO:0042277 ISO
    cobalt ion binding GO:0050897 ISO
    metalloaminopeptidase activity GO:0070006 IBA
    metalloaminopeptidase activity GO:0070006 ISO
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IEA
    protein processing GO:0016485 ISO
    negative regulation of blood pressure GO:0045776 ISO
Subcellular Localization
    extracellular region GO:0005576 ISO
    extracellular region GO:0005576 ISS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    external side of plasma membrane GO:0009897 ISO
    secretory granule GO:0030141 ISO
 Experiment description of studies that identified Rnpep in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rnpep
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Eed  
Affinity Capture-MS Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Rnpep is involved
No pathways found





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