Gene description for Hk3
Gene name hexokinase 3
Gene symbol Hk3
Other names/aliases HK III
HK-III
Species Mus musculus
 Database cross references - Hk3
ExoCarta ExoCarta_212032
Vesiclepedia VP_212032
Entrez Gene 212032
UniProt Q3TRM8  
 Hk3 identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Hk3
Molecular Function
    glucokinase activity GO:0004340 IBA
    glucokinase activity GO:0004340 ISO
    glucokinase activity GO:0004340 ISS
    hexokinase activity GO:0004396 ISO
    ATP binding GO:0005524 IEA
    D-glucose binding GO:0005536 IEA
    fructokinase activity GO:0008865 IBA
    fructokinase activity GO:0008865 ISO
    fructokinase activity GO:0008865 ISS
    mannokinase activity GO:0019158 IBA
    enzyme binding GO:0019899 ISO
    hormone binding GO:0042562 ISO
Biological Process
    intracellular glucose homeostasis GO:0001678 IBA
    intracellular glucose homeostasis GO:0001678 IEA
    fructose 6-phosphate metabolic process GO:0006002 ISO
    fructose 6-phosphate metabolic process GO:0006002 ISS
    glucose metabolic process GO:0006006 IBA
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 IEA
    hexose metabolic process GO:0019318 IEA
    carbohydrate phosphorylation GO:0046835 IEA
    glucose 6-phosphate metabolic process GO:0051156 IBA
    glucose 6-phosphate metabolic process GO:0051156 ISO
    glucose 6-phosphate metabolic process GO:0051156 ISS
    negative regulation of hydrogen peroxide-mediated programmed cell death GO:1901299 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 IBA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Hk3 in sEVs
1
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Hk3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
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