Gene description for Hk3
Gene name hexokinase 3
Gene symbol Hk3
Other names/aliases HK III
HK-III
Species Mus musculus
 Database cross references - Hk3
ExoCarta ExoCarta_212032
Vesiclepedia VP_212032
Entrez Gene 212032
UniProt Q3TRM8  
 Hk3 identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Hk3
Molecular Function
    glucokinase activity GO:0004340 IBA
    glucokinase activity GO:0004340 ISO
    glucokinase activity GO:0004340 ISS
    hexokinase activity GO:0004396 ISO
    ATP binding GO:0005524 IEA
    D-glucose binding GO:0005536 IEA
    fructokinase activity GO:0008865 IBA
    fructokinase activity GO:0008865 ISO
    fructokinase activity GO:0008865 ISS
    mannokinase activity GO:0019158 IBA
    enzyme binding GO:0019899 ISO
    hormone binding GO:0042562 ISO
Biological Process
    intracellular glucose homeostasis GO:0001678 IBA
    intracellular glucose homeostasis GO:0001678 IEA
    fructose 6-phosphate metabolic process GO:0006002 ISO
    fructose 6-phosphate metabolic process GO:0006002 ISS
    glucose metabolic process GO:0006006 IBA
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 IEA
    hexose metabolic process GO:0019318 IEA
    carbohydrate phosphorylation GO:0046835 IEA
    glucose 6-phosphate metabolic process GO:0051156 IBA
    glucose 6-phosphate metabolic process GO:0051156 ISO
    glucose 6-phosphate metabolic process GO:0051156 ISS
    negative regulation of hydrogen peroxide-mediated programmed cell death GO:1901299 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 IBA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Hk3 in sEVs
1
Experiment ID 214
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Hk3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
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