Gene description for Camkk2
Gene name calcium/calmodulin-dependent protein kinase kinase 2, beta
Gene symbol Camkk2
Other names/aliases 6330570N16Rik
AW061083
mKIAA0787
Species Mus musculus
 Database cross references - Camkk2
ExoCarta ExoCarta_207565
Vesiclepedia VP_207565
Entrez Gene 207565
UniProt Q8C078  
 Camkk2 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Camkk2
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    calcium/calmodulin-dependent protein kinase activity GO:0004683 IEA
    calcium/calmodulin-dependent protein kinase activity GO:0004683 ISO
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    calmodulin binding GO:0005516 ISO
    ATP binding GO:0005524 IEA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    positive regulation of protein phosphorylation GO:0001934 ISO
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISS
    protein autophosphorylation GO:0046777 ISO
    protein autophosphorylation GO:0046777 ISS
    CAMKK-AMPK signaling cascade GO:0061762 IBA
    CAMKK-AMPK signaling cascade GO:0061762 IEA
    CAMKK-AMPK signaling cascade GO:0061762 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    neuron projection GO:0043005 IEA
 Experiment description of studies that identified Camkk2 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Camkk2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Slc25a11  
Co-fractionation Mus musculus
2 Agap2  
Affinity Capture-MS Mus musculus
3 Atg5  
Affinity Capture-RNA Mus musculus
4 Fanci  
Co-fractionation Mus musculus
View the network image/svg+xml



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