Gene description for Spr
Gene name sepiapterin reductase
Gene symbol Spr
Other names/aliases AA409688
ENSMUSG00000071345
Gm10328
Species Mus musculus
 Database cross references - Spr
ExoCarta ExoCarta_20751
Vesiclepedia VP_20751
Entrez Gene 20751
 Spr identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Spr
Molecular Function
    sepiapterin reductase (NADP+) activity GO:0004757 IBA
    sepiapterin reductase (NADP+) activity GO:0004757 ISO
    sepiapterin reductase (NADP+) activity GO:0004757 ISS
    protein homodimerization activity GO:0042803 IPI
Biological Process
    L-phenylalanine metabolic process GO:0006558 IMP
    tetrahydrobiopterin biosynthetic process GO:0006729 IBA
    tetrahydrobiopterin biosynthetic process GO:0006729 IEA
    nitric oxide biosynthetic process GO:0006809 ISO
    pteridine metabolic process GO:0019889 IMP
    regulation of multicellular organism growth GO:0040014 IMP
    norepinephrine metabolic process GO:0042415 IMP
    dopamine metabolic process GO:0042417 IMP
    serotonin metabolic process GO:0042428 IMP
    tetrahydrobiopterin metabolic process GO:0046146 IMP
    cell morphogenesis involved in neuron differentiation GO:0048667 IMP
    voluntary musculoskeletal movement GO:0050882 IMP
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Spr in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Spr
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Spr is involved
No pathways found





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