Gene description for Spn
Gene name sialophorin
Gene symbol Spn
Other names/aliases A630014B01Rik
Cd43
Galgp
Ly-48
Ly48
Species Mus musculus
 Database cross references - Spn
ExoCarta ExoCarta_20737
Vesiclepedia VP_20737
Entrez Gene 20737
UniProt P15702  
 Spn identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Spn
Molecular Function
    transmembrane signaling receptor activity GO:0004888 IEA
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 ISO
    Hsp70 protein binding GO:0030544 IEA
    Hsp70 protein binding GO:0030544 ISO
    heat shock protein binding GO:0031072 IBA
    heat shock protein binding GO:0031072 IPI
    heat shock protein binding GO:0031072 ISO
Biological Process
    response to protozoan GO:0001562 IDA
    negative regulation of type IV hypersensitivity GO:0001808 IMP
    positive regulation of protein phosphorylation GO:0001934 IGI
    T-helper 1 cell lineage commitment GO:0002296 ISO
    T-helper 1 cell lineage commitment GO:0002296 ISS
    protein phosphorylation GO:0006468 IGI
    negative regulation of cell adhesion GO:0007162 IDA
    negative regulation of cell adhesion GO:0007162 IMP
    cell surface receptor signaling pathway GO:0007166 IBA
    cell surface receptor signaling pathway GO:0007166 IDA
    T cell costimulation GO:0031295 IDA
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    T cell proliferation GO:0042098 IDA
    positive regulation of T cell proliferation GO:0042102 IDA
    negative regulation of T cell proliferation GO:0042130 IMP
    negative regulation of T cell proliferation GO:0042130 IMP
    defense response to bacterium GO:0042742 IBA
    defense response to bacterium GO:0042742 IMP
    defense response to bacterium GO:0042742 ISO
    negative thymic T cell selection GO:0045060 IMP
    regulation of defense response to virus GO:0050688 IMP
    regulation of immune response GO:0050776 IBA
    regulation of immune response GO:0050776 IMP
    negative regulation of T cell activation GO:0050868 IMP
    leukocyte tethering or rolling GO:0050901 IMP
    apoptotic signaling pathway GO:0097190 IDA
    regulation of T cell migration GO:2000404 IMP
    positive regulation of T cell migration GO:2000406 IMP
Subcellular Localization
    uropod GO:0001931 IDA
    basement membrane GO:0005604 IDA
    extracellular space GO:0005615 ISO
    plasma membrane GO:0005886 IDA
    microvillus GO:0005902 ISO
    microvillus GO:0005902 ISS
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 ISO
    PML body GO:0016605 IEA
    cleavage furrow GO:0032154 ISO
 Experiment description of studies that identified Spn in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Spn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Spn is involved
PathwayEvidenceSource
Basigin interactions IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Hemostasis IEA Reactome





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