Gene description for Slc2a1
Gene name solute carrier family 2 (facilitated glucose transporter), member 1
Gene symbol Slc2a1
Other names/aliases Glut-1
Glut1
Species Mus musculus
 Database cross references - Slc2a1
ExoCarta ExoCarta_20525
Vesiclepedia VP_20525
Entrez Gene 20525
UniProt P17809  
 Slc2a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc2a1
Molecular Function
    long-chain fatty acid transmembrane transporter activity GO:0005324 IEA
    long-chain fatty acid transmembrane transporter activity GO:0005324 ISO
    protein binding GO:0005515 IPI
    fucose transmembrane transporter activity GO:0015150 IDA
    kinase binding GO:0019900 IEA
    kinase binding GO:0019900 ISO
    dehydroascorbic acid transmembrane transporter activity GO:0033300 IDA
    dehydroascorbic acid transmembrane transporter activity GO:0033300 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    xenobiotic transmembrane transporter activity GO:0042910 IEA
    xenobiotic transmembrane transporter activity GO:0042910 ISO
    D-glucose transmembrane transporter activity GO:0055056 IBA
    D-glucose transmembrane transporter activity GO:0055056 IDA
    D-glucose transmembrane transporter activity GO:0055056 ISO
    D-glucose transmembrane transporter activity GO:0055056 ISS
Biological Process
    response to hypoxia GO:0001666 IEA
    central nervous system development GO:0007417 ISO
    female pregnancy GO:0007565 IEA
    fucose transmembrane transport GO:0015756 IEA
    long-chain fatty acid import across plasma membrane GO:0015911 IEA
    long-chain fatty acid import across plasma membrane GO:0015911 ISO
    cerebral cortex development GO:0021987 IEA
    response to insulin GO:0032868 IBA
    response to insulin GO:0032868 IEA
    cellular response to glucose starvation GO:0042149 IDA
    xenobiotic transport GO:0042908 IEA
    xenobiotic transport GO:0042908 ISO
    photoreceptor cell maintenance GO:0045494 ISS
    D-glucose import GO:0046323 IBA
    D-glucose import GO:0046323 IEA
    D-glucose import GO:0046323 ISO
    protein-containing complex assembly GO:0065003 ISO
    protein-containing complex assembly GO:0065003 ISS
    dehydroascorbic acid transport GO:0070837 IBA
    dehydroascorbic acid transport GO:0070837 IDA
    dehydroascorbic acid transport GO:0070837 ISO
    cellular response to mechanical stimulus GO:0071260 IEA
    cellular hyperosmotic response GO:0071474 IEA
    D-glucose import across plasma membrane GO:0098708 IEA
    D-glucose import across plasma membrane GO:0098708 ISO
    transport across blood-brain barrier GO:0150104 IEA
    transport across blood-brain barrier GO:0150104 ISO
    transport across blood-brain barrier GO:0150104 ISO
    response to Thyroglobulin triiodothyronine GO:1904016 IEA
    D-glucose transmembrane transport GO:1904659 IDA
    D-glucose transmembrane transport GO:1904659 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    female germ cell nucleus GO:0001674 IDA
    photoreceptor inner segment GO:0001917 IDA
    female pronucleus GO:0001939 IDA
    intracellular anatomical structure GO:0005622 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    caveola GO:0005901 IEA
    caveola GO:0005901 ISO
    cell-cell junction GO:0005911 ISO
    intercalated disc GO:0014704 IEA
    intercalated disc GO:0014704 ISO
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    Z disc GO:0030018 IEA
    Z disc GO:0030018 ISO
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    cortical actin cytoskeleton GO:0030864 ISO
    cortical actin cytoskeleton GO:0030864 ISS
    vesicle GO:0031982 IDA
    sarcolemma GO:0042383 IEA
    sarcolemma GO:0042383 ISO
    membrane raft GO:0045121 IDA
    synapse GO:0045202 IDA
    presynapse GO:0098793 IDA
    glucose transporter complex GO:1990350 ISO
 Experiment description of studies that identified Slc2a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc2a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
3 Kcna3  
Affinity Capture-MS Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 Mapt  
Affinity Capture-MS Mus musculus
6 Atg5  
Affinity Capture-MS Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
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