Gene description for Slc22a3
Gene name solute carrier family 22 (organic cation transporter), member 3
Gene symbol Slc22a3
Other names/aliases EMT
Oct3
Orct3
Slca22a3
Species Mus musculus
 Database cross references - Slc22a3
ExoCarta ExoCarta_20519
Vesiclepedia VP_20519
Entrez Gene 20519
UniProt Q9WTW5  
 Slc22a3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc22a3
Molecular Function
    neurotransmitter transmembrane transporter activity GO:0005326 IMP
    neurotransmitter transmembrane transporter activity GO:0005326 ISO
    dopamine:sodium symporter activity GO:0005330 IEA
    dopamine:sodium symporter activity GO:0005330 ISO
    monoamine transmembrane transporter activity GO:0008504 IMP
    monoamine transmembrane transporter activity GO:0008504 ISO
    organic anion transmembrane transporter activity GO:0008514 IEA
    organic anion transmembrane transporter activity GO:0008514 ISO
    organic cation transmembrane transporter activity GO:0015101 IBA
    organic cation transmembrane transporter activity GO:0015101 IDA
    organic cation transmembrane transporter activity GO:0015101 ISO
    spermidine transmembrane transporter activity GO:0015606 ISO
    spermidine transmembrane transporter activity GO:0015606 ISS
    quaternary ammonium group transmembrane transporter activity GO:0015651 IDA
    quaternary ammonium group transmembrane transporter activity GO:0015651 ISO
    toxin transmembrane transporter activity GO:0019534 IEA
    toxin transmembrane transporter activity GO:0019534 ISO
    transmembrane transporter activity GO:0022857 IDA
Biological Process
    monoatomic ion transport GO:0006811 IEA
    neurotransmitter transport GO:0006836 IMP
    neurotransmitter transport GO:0006836 ISO
    serotonin transport GO:0006837 IMP
    serotonin transport GO:0006837 ISO
    organic cation transport GO:0015695 IBA
    organic cation transport GO:0015695 IDA
    organic cation transport GO:0015695 ISO
    organic cation transport GO:0015695 ISO
    quaternary ammonium group transport GO:0015697 IDA
    quaternary ammonium group transport GO:0015697 ISO
    quaternary ammonium group transport GO:0015697 ISO
    organic anion transport GO:0015711 ISO
    monocarboxylic acid transport GO:0015718 IMP
    monoamine transport GO:0015844 IMP
    monoamine transport GO:0015844 ISO
    spermidine transport GO:0015848 ISO
    spermidine transport GO:0015848 ISS
    organic hydroxy compound transport GO:0015850 IMP
    dopamine transport GO:0015872 IMP
    dopamine transport GO:0015872 ISO
    norepinephrine transport GO:0015874 IMP
    norepinephrine transport GO:0015874 ISO
    norepinephrine transport GO:0015874 ISS
    regulation of appetite GO:0032098 IMP
    xenobiotic transport GO:0042908 IEA
    xenobiotic transport GO:0042908 ISO
    epinephrine transport GO:0048241 IEA
    epinephrine transport GO:0048241 ISO
    histamine transport GO:0051608 IMP
    histamine transport GO:0051608 IMP
    histamine transport GO:0051608 ISO
    histamine transport GO:0051608 ISS
    serotonin uptake GO:0051610 IEA
    serotonin uptake GO:0051610 ISO
    histamine uptake GO:0051615 IMP
    histamine uptake GO:0051615 ISO
    norepinephrine uptake GO:0051620 IEA
    norepinephrine uptake GO:0051620 ISO
    epinephrine uptake GO:0051625 IEA
    epinephrine uptake GO:0051625 ISO
    establishment of localization in cell GO:0051649 IMP
    dopamine uptake GO:0090494 IEA
    dopamine uptake GO:0090494 ISO
    spermidine transmembrane transport GO:1903711 IEA
    cellular detoxification GO:1990748 IEA
    cellular detoxification GO:1990748 ISO
Subcellular Localization
    nuclear outer membrane GO:0005640 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    endomembrane system GO:0012505 IDA
    endomembrane system GO:0012505 ISO
    basolateral plasma membrane GO:0016323 ISO
    basolateral plasma membrane GO:0016323 ISS
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    apical plasma membrane GO:0016324 ISS
    mitochondrial membrane GO:0031966 IDA
    mitochondrial membrane GO:0031966 ISO
    neuronal cell body GO:0043025 IDA
    presynapse GO:0098793 IEA
 Experiment description of studies that identified Slc22a3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc22a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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