Gene description for Slc12a7
Gene name solute carrier family 12, member 7
Gene symbol Slc12a7
Other names/aliases AA408796
D13Ertd261e
Kcc4
Species Mus musculus
 Database cross references - Slc12a7
ExoCarta ExoCarta_20499
Vesiclepedia VP_20499
Entrez Gene 20499
UniProt Q9WVL3  
 Slc12a7 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc12a7
Molecular Function
    potassium:chloride symporter activity GO:0015379 IBA
    potassium:chloride symporter activity GO:0015379 IDA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    cell volume homeostasis GO:0006884 IBA
    cell volume homeostasis GO:0006884 ISO
    chemical synaptic transmission GO:0007268 IBA
    chloride ion homeostasis GO:0055064 IBA
    potassium ion homeostasis GO:0055075 IBA
    cellular response to glucose stimulus GO:0071333 IDA
    potassium ion transmembrane transport GO:0071805 IDA
    chloride transmembrane transport GO:1902476 IBA
    chloride transmembrane transport GO:1902476 IEA
    potassium ion import across plasma membrane GO:1990573 IBA
    potassium ion import across plasma membrane GO:1990573 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Slc12a7 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc12a7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Slc12a5  
Co-fractionation Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Slc12a4  
Co-fractionation Mus musculus
View the network image/svg+xml



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