Gene description for Nptn
Gene name neuroplastin
Gene symbol Nptn
Other names/aliases AW554172
SDR-1
Sdfr1
Species Mus musculus
 Database cross references - Nptn
ExoCarta ExoCarta_20320
Vesiclepedia VP_20320
Entrez Gene 20320
UniProt P97300  
 Nptn identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nptn
Molecular Function
    type 1 fibroblast growth factor receptor binding GO:0005105 ISO
    type 1 fibroblast growth factor receptor binding GO:0005105 ISS
    protein binding GO:0005515 IPI
    transmembrane transporter binding GO:0044325 ISO
    cell adhesion molecule binding GO:0050839 ISO
    cell adhesion molecule binding GO:0050839 ISS
    cell-cell adhesion mediator activity GO:0098632 IBA
Biological Process
    negative regulation of cytokine production GO:0001818 IMP
    positive regulation of protein phosphorylation GO:0001934 ISO
    positive regulation of protein phosphorylation GO:0001934 ISS
    intracellular calcium ion homeostasis GO:0006874 IMP
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IBA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 ISO
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 ISS
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISO
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISS
    axon guidance GO:0007411 IBA
    visual learning GO:0008542 ISO
    positive regulation of neuron projection development GO:0010976 ISO
    positive regulation of neuron projection development GO:0010976 ISS
    positive regulation of fibroblast growth factor receptor signaling pathway GO:0045743 ISO
    positive regulation of fibroblast growth factor receptor signaling pathway GO:0045743 ISS
    positive regulation of long-term neuronal synaptic plasticity GO:0048170 ISO
    positive regulation of long-term neuronal synaptic plasticity GO:0048170 ISS
    modulation of chemical synaptic transmission GO:0050804 IMP
    synapse organization GO:0050808 IMP
    long-term synaptic potentiation GO:0060291 ISO
    long-term synaptic potentiation GO:0060291 ISS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    dendrite self-avoidance GO:0070593 IBA
    trans-synaptic signaling by trans-synaptic complex, modulating synaptic transmission GO:0099557 ISO
    positive regulation of long-term synaptic potentiation GO:1900273 ISO
    regulation of receptor localization to synapse GO:1902683 IMP
    positive regulation of protein localization GO:1903829 ISO
    excitatory synapse assembly GO:1904861 IMP
Subcellular Localization
    immunological synapse GO:0001772 IDA
    plasma membrane GO:0005886 IBA
    cell surface GO:0009986 ISO
    postsynaptic density GO:0014069 IEA
    axon GO:0030424 IBA
    dendrite GO:0030425 ISO
    presynaptic membrane GO:0042734 ISO
    presynaptic membrane GO:0042734 ISS
    presynaptic active zone membrane GO:0048787 ISO
    inhibitory synapse GO:0060077 ISO
    synaptic membrane GO:0097060 ISO
    synaptic membrane GO:0097060 ISO
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    postsynaptic density membrane GO:0098839 ISO
    glutamatergic synapse GO:0098978 ISO
    GABA-ergic synapse GO:0098982 ISO
 Experiment description of studies that identified Nptn in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nptn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Uqcrh  
Co-fractionation Mus musculus
2 Lnx1  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Sstr3  
Proximity Label-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Syp  
Co-fractionation Mus musculus
7 Sppl2a  
Co-fractionation Mus musculus
8 Agap2  
Affinity Capture-MS Mus musculus
9 Huwe1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Nptn is involved
No pathways found





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