Gene description for Rrm1
Gene name ribonucleotide reductase M1
Gene symbol Rrm1
Other names/aliases RnrM1
Species Mus musculus
 Database cross references - Rrm1
ExoCarta ExoCarta_20133
Vesiclepedia VP_20133
Entrez Gene 20133
UniProt P07742  
 Rrm1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rrm1
Molecular Function
    ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor GO:0004748 IDA
    ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor GO:0004748 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 IEA
    purine nucleotide binding GO:0017076 IDA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    ribonucleoside-diphosphate reductase activity GO:0061731 ISO
    disordered domain specific binding GO:0097718 IPI
Biological Process
    DNA synthesis involved in DNA repair GO:0000731 NAS
    pyrimidine nucleobase metabolic process GO:0006206 IEA
    mitochondrial DNA replication GO:0006264 IMP
    mitochondrial DNA replication GO:0006264 ISO
    DNA repair GO:0006281 ISO
    male gonad development GO:0008584 IEA
    ribonucleoside diphosphate metabolic process GO:0009185 IDA
    ribonucleoside diphosphate metabolic process GO:0009185 ISO
    deoxyribonucleotide biosynthetic process GO:0009263 IBA
    deoxyribonucleotide biosynthetic process GO:0009263 IDA
    2'-deoxyribonucleotide biosynthetic process GO:0009265 IDA
    2'-deoxyribonucleotide biosynthetic process GO:0009265 ISO
    response to ionizing radiation GO:0010212 IEA
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 ISO
    cell proliferation in forebrain GO:0021846 IEA
    protein heterotetramerization GO:0051290 IPI
    retina development in camera-type eye GO:0060041 IEA
    positive regulation of G0 to G1 transition GO:0070318 IDA
    positive regulation of G0 to G1 transition GO:0070318 ISO
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISO
Subcellular Localization
    nuclear envelope GO:0005635 IEA
    nuclear envelope GO:0005635 ISO
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 ISO
    ribonucleoside-diphosphate reductase complex GO:0005971 IBA
    ribonucleoside-diphosphate reductase complex GO:0005971 IDA
    ribonucleoside-diphosphate reductase complex GO:0005971 IMP
    ribonucleoside-diphosphate reductase complex GO:0005971 ISO
    ribonucleoside-diphosphate reductase complex GO:0005971 ISS
    cell projection GO:0042995 IEA
    cell projection GO:0042995 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
 Experiment description of studies that identified Rrm1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rrm1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ABCG1  
Affinity Capture-MS Homo sapiens
2 Lrif1  
Co-fractionation Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Kat5  
Reconstituted Complex Mus musculus
View the network image/svg+xml
 Pathways in which Rrm1 is involved
PathwayEvidenceSource
Interconversion of nucleotide di- and triphosphates IEA Reactome
Metabolism IEA Reactome
Metabolism of nucleotides IEA Reactome





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