|
 Gene description for Rras |
| Gene name |
Harvey rat sarcoma oncogene, subgroup R |
| Gene symbol |
Rras |
| Other names/aliases |
AI573426 |
| Species |
Mus musculus |
| Database cross references - Rras |
| ExoCarta |
ExoCarta_20130 |
| Vesiclepedia |
VP_20130 |
| Entrez Gene |
20130 |
| UniProt |
P10833
|
| Rras identified in sEVs derived from the following tissue/cell type |
|
Colon cancer cells
|
37309723
|
|
Mov neuroglial cells
|
15210972
|
|
T-cell lymphoma cells
|
37309723
|
| Gene ontology annotations for Rras |
|
|
| Experiment description of studies that identified Rras in sEVs |
| 1 |
| Experiment ID |
907 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Colon cancer cells |
| Sample name |
C26 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 2 |
| Experiment ID |
39 |
| MISEV standards |
|
✔
IEM
|
Biophysical techniques |
|
✔
TSG101|HSC70|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
15210972
|
| Organism |
Mus musculus |
| Experiment description |
Cells release prions in association with exosomes. |
| Authors |
"Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G" |
| Journal name |
PNAS
|
| Publication year |
2004 |
| Sample |
Mov neuroglial cells |
| Sample name |
Mov neuroglial cell |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.14 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy |
|
|
| 3 |
| Experiment ID |
908 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
T-cell lymphoma cells |
| Sample name |
EL4 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| Protein-protein interactions for Rras |
|
|
| Pathways in which Rras is involved |
|
|
|
