Gene description for Rgn
Gene name regucalcin
Gene symbol Rgn
Other names/aliases AI265316
GNL
SMP30
Species Mus musculus
 Database cross references - Rgn
ExoCarta ExoCarta_19733
Vesiclepedia VP_19733
Entrez Gene 19733
UniProt Q64374  
 Rgn identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rgn
Molecular Function
    gluconolactonase activity GO:0004341 IBA
    gluconolactonase activity GO:0004341 IDA
    gluconolactonase activity GO:0004341 IMP
    gluconolactonase activity GO:0004341 ISO
    gluconolactonase activity GO:0004341 ISS
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    enzyme regulator activity GO:0030234 IEA
Biological Process
    kidney development GO:0001822 IEA
    negative regulation of protein phosphorylation GO:0001933 ISO
    intracellular calcium ion homeostasis GO:0006874 ISO
    intracellular calcium ion homeostasis GO:0006874 ISS
    spermatogenesis GO:0007283 IEA
    spermatogenesis GO:0007283 ISO
    positive regulation of triglyceride biosynthetic process GO:0010867 IEA
    positive regulation of triglyceride biosynthetic process GO:0010867 ISO
    positive regulation of glucose metabolic process GO:0010907 IEA
    positive regulation of glucose metabolic process GO:0010907 ISO
    L-ascorbic acid biosynthetic process GO:0019853 IBA
    L-ascorbic acid biosynthetic process GO:0019853 IDA
    L-ascorbic acid biosynthetic process GO:0019853 IMP
    positive regulation of ATP-dependent activity GO:0032781 ISS
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of nitric oxide biosynthetic process GO:0045019 IEA
    negative regulation of nitric oxide biosynthetic process GO:0045019 ISO
    positive regulation of fatty acid biosynthetic process GO:0045723 IEA
    positive regulation of fatty acid biosynthetic process GO:0045723 ISO
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    negative regulation of epithelial cell proliferation GO:0050680 ISO
    regulation of calcium-mediated signaling GO:0050848 ISO
    regulation of calcium-mediated signaling GO:0050848 ISS
    liver regeneration GO:0097421 IEA
    negative regulation of flagellated sperm motility GO:1901318 IEA
    negative regulation of flagellated sperm motility GO:1901318 ISO
    negative regulation of RNA biosynthetic process GO:1902679 IEA
    negative regulation of RNA biosynthetic process GO:1902679 ISO
    negative regulation of bone development GO:1903011 IEA
    negative regulation of bone development GO:1903011 ISO
    positive regulation of proteolysis involved in protein catabolic process GO:1903052 IEA
    positive regulation of proteolysis involved in protein catabolic process GO:1903052 ISO
    negative regulation of DNA catabolic process GO:1903625 IEA
    negative regulation of DNA catabolic process GO:1903625 ISO
    negative regulation of DNA biosynthetic process GO:2000279 IEA
    negative regulation of DNA biosynthetic process GO:2000279 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified Rgn in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rgn
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rgn is involved
No pathways found





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