Gene description for Rac2
Gene name RAS-related C3 botulinum substrate 2
Gene symbol Rac2
Other names/aliases AI323801
AI452260
Species Mus musculus
 Database cross references - Rac2
ExoCarta ExoCarta_19354
Vesiclepedia VP_19354
Entrez Gene 19354
UniProt Q05144  
 Rac2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rac2
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    protein kinase regulator activity GO:0019887 IMP
    protein kinase binding GO:0019901 IBA
Biological Process
    chemotaxis GO:0006935 IBA
    chemotaxis GO:0006935 IDA
    actin filament organization GO:0007015 IBA
    actin filament organization GO:0007015 IEA
    actin filament organization GO:0007015 ISO
    establishment or maintenance of cell polarity GO:0007163 IBA
    signal transduction GO:0007165 IBA
    G protein-coupled receptor signaling pathway GO:0007186 IGI
    small GTPase-mediated signal transduction GO:0007264 IEA
    cell population proliferation GO:0008283 IGI
    cell population proliferation GO:0008283 IMP
    regulation of cell shape GO:0008360 IBA
    positive regulation of lamellipodium assembly GO:0010592 IBA
    positive regulation of lamellipodium assembly GO:0010592 IMP
    positive regulation of lamellipodium assembly GO:0010592 ISO
    regulation of cell-substrate adhesion GO:0010810 IEA
    regulation of cell-substrate adhesion GO:0010810 ISO
    cell projection assembly GO:0030031 IDA
    actin cytoskeleton organization GO:0030036 IDA
    actin cytoskeleton organization GO:0030036 ISO
    cortical cytoskeleton organization GO:0030865 IBA
    regulation of actin cytoskeleton organization GO:0032956 IBA
    regulation of T cell proliferation GO:0042129 IMP
    erythrocyte enucleation GO:0043131 IMP
    regulation of mast cell degranulation GO:0043304 IMP
    bone resorption GO:0045453 IEA
    bone resorption GO:0045453 ISO
    regulation of respiratory burst GO:0060263 IEA
    regulation of respiratory burst GO:0060263 ISO
    regulation of mast cell chemotaxis GO:0060753 IMP
    mast cell proliferation GO:0070662 IGI
    mast cell proliferation GO:0070662 IMP
    positive regulation of mast cell proliferation GO:0070668 IGI
    positive regulation of mast cell proliferation GO:0070668 IMP
    lymphocyte aggregation GO:0071593 IEA
    lymphocyte aggregation GO:0071593 ISO
    positive regulation of neutrophil chemotaxis GO:0090023 IMP
    positive regulation of neutrophil chemotaxis GO:0090023 ISO
    regulation of neutrophil migration GO:1902622 IBA
    regulation of neutrophil migration GO:1902622 ISO
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytoskeleton GO:0005856 IBA
    actin filament GO:0005884 IEA
    actin filament GO:0005884 ISO
    plasma membrane GO:0005886 IBA
    membrane GO:0016020 IDA
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    cytoplasmic vesicle GO:0031410 IBA
    cell projection GO:0042995 IBA
 Experiment description of studies that identified Rac2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rac2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMAD4  
Affinity Capture-Luminescence Homo sapiens
2 Fancd2  
Affinity Capture-MS Mus musculus
3 Foxp3  
Affinity Capture-MS Mus musculus
4 Usp7  
Affinity Capture-MS Mus musculus
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