Gene description for Rab7
Gene name RAB7, member RAS oncogene family
Gene symbol Rab7
Other names/aliases Rab7a
Species Mus musculus
 Database cross references - Rab7
ExoCarta ExoCarta_19349
Entrez Gene 19349
UniProt P51150  
 Rab7 identified in exosomes derived from the following tissue/cell type
Dendritic cells 11390481    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Microglia 16081791    
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
 Gene ontology annotations for Rab7
Molecular Function
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    GTPase activity GO:0003924 ISO
    Rac GTPase binding GO:0048365 ISO
    GTP binding GO:0005525 ISO
    GDP binding GO:0019003 ISO
Biological Process
    transport GO:0006810 IEA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    early endosome to late endosome transport GO:0045022 ISO
    bone resorption GO:0045453 ISO
    protein transport GO:0015031 IEA
    positive regulation of exosomal secretion GO:1903543 ISO
    positive regulation of viral process GO:0048524 ISO
    phagosome-lysosome fusion GO:0090385 ISO
    viral release from host cell GO:0019076 ISO
    endosome to lysosome transport GO:0008333 ISO
    small GTPase mediated signal transduction GO:0007264 IEA
    protein targeting to lysosome GO:0006622 ISO
    intracellular protein transport GO:0006886 TAS
    protein to membrane docking GO:0022615 ISO
    positive regulation of protein catabolic process GO:0045732 ISO
    epidermal growth factor catabolic process GO:0007174 ISO
    regulation of autophagosome assembly GO:2000785 ISO
    Rab protein signal transduction GO:0032482 IBA
    phagosome acidification GO:0090383 ISO
Subcellular Localization
    intracellular GO:0005622 TAS
    cytoplasmic vesicle GO:0031410 ISO
    phagocytic vesicle GO:0045335 ISO
    extracellular exosome GO:0070062 ISO
    terminal bouton GO:0043195 ISO
    alveolar lamellar body GO:0097208 ISO
    retromer complex GO:0030904 ISO
    Golgi apparatus GO:0005794 IDA
    pre-autophagosomal structure membrane GO:0034045 IDA
    membrane GO:0016020 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    endosome GO:0005768 IEA
    cytoplasm GO:0005737 IDA
    late endosome GO:0005770 ISO
    cell GO:0005623 TAS
    vacuolar membrane GO:0005774 IBA
    lysosome GO:0005764 ISO
 Experiment description of studies that identified Rab7 in exosomes
1
Experiment ID 10
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD9|MHCII|MHCI|CD86|LAMP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S.
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 73
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 14
ISEV standards
✔
EM
EV Biophysical techniques
✔
RAB7|RAB11
EV Cytosolic markers
✔
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
✔
DNM
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
5
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
6
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rab7
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rab7 is involved
PathwayEvidenceSource
MHC class II antigen presentation IEA Reactome





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