Gene description for Rab12
Gene name RAB12, member RAS oncogene family
Gene symbol Rab12
Other names/aliases 2900054P15Rik
AW555448
C77700
Species Mus musculus
 Database cross references - Rab12
ExoCarta ExoCarta_19328
Vesiclepedia VP_19328
Entrez Gene 19328
UniProt P35283  
 Rab12 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Rab12
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTP binding GO:0005525 IEA
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
Biological Process
    exocytosis GO:0006887 IBA
    autophagy GO:0006914 IEA
    endosome to lysosome transport GO:0008333 IMP
    protein transport GO:0015031 IEA
    positive regulation of macroautophagy GO:0016239 IMP
    protein catabolic process GO:0030163 IMP
    endocytic recycling GO:0032456 IBA
    Rab protein signal transduction GO:0032482 IEA
    cellular response to type II interferon GO:0071346 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IEA
    endosome GO:0005768 IBA
    autophagosome GO:0005776 IDA
    plasma membrane GO:0005886 IBA
    synaptic vesicle GO:0008021 IBA
    trans-Golgi network transport vesicle GO:0030140 IBA
    secretory granule GO:0030141 ISO
    phagocytic vesicle GO:0045335 IDA
    recycling endosome membrane GO:0055038 IDA
 Experiment description of studies that identified Rab12 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Rab12
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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