Gene description for Ptrf
Gene name polymerase I and transcript release factor
Gene symbol Ptrf
Other names/aliases 2310075E07Rik
AW546441
Cav-p60
Cavin
Species Mus musculus
 Database cross references - Ptrf
ExoCarta ExoCarta_19285
Vesiclepedia VP_19285
Entrez Gene 19285
UniProt O54724  
 Ptrf identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ptrf
Molecular Function
    protein binding GO:0005515 IPI
    rRNA primary transcript binding GO:0042134 IBA
    rRNA primary transcript binding GO:0042134 IDA
    rRNA primary transcript binding GO:0042134 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    transcription initiation at RNA polymerase I promoter GO:0006361 IBA
    transcription initiation at RNA polymerase I promoter GO:0006361 IDA
    transcription initiation at RNA polymerase I promoter GO:0006361 ISO
    termination of RNA polymerase I transcription GO:0006363 IBA
    termination of RNA polymerase I transcription GO:0006363 IDA
    termination of RNA polymerase I transcription GO:0006363 IDA
    termination of RNA polymerase I transcription GO:0006363 ISO
    rRNA transcription GO:0009303 IMP
    protein secretion GO:0009306 IMP
    positive regulation of cell motility GO:2000147 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 ISO
    caveola GO:0005901 IBA
    caveola GO:0005901 IDA
    caveola GO:0005901 ISO
    caveola GO:0005901 ISS
    protein-containing complex GO:0032991 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    membrane raft GO:0045121 ISO
 Experiment description of studies that identified Ptrf in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ptrf
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
2 Xdh  
Co-fractionation Mus musculus
3 Htt  
Affinity Capture-MS Mus musculus
4 Rab11fip5  
Co-fractionation Mus musculus
5 Mipep  
Co-fractionation Mus musculus
6 Arsb  
Co-fractionation Mus musculus
7 Dmd  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml



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